Human Anti-High mannose glycans Recombinant Antibody (clone 2G12) (CAT#: HPAB-S0061-YC)

Figure 1 Deposition of complement factor C3 on CEM. NKR cells infected with HIV-1MN.

(A) Control staining with active complement in the absence of antibody; (B) MAb 2G12 with active complement; (C) MAb 3D6 with active complement (negative control); (D) HIV1 hIgG with active complement.

Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.

Figure 2 ADCC mediated by MAb 2G12.

MAb 2G12 is a potent mediator of ADCC as it induces specific lysis of three of the tested targets (gp120/IIIB, IIIB, and MN) at concentrations of , 1 μg/ml.

Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.

Figure 3 Effect of sCD4 on 2G12 and 1B1 binding to gp120.

MAbs 2G12 (●) and 1B1 (▲) were bound to gp160 coated onto ELISA plates, and then incubation with sCD4 and detection of bound sCD4 by the murine anti-CD4 MAb OKT4 were performed. The mean binding value (mean of four replicate wells) obtained with sCD4 in the absence of MAb is represented by the dashed line. While human MAb 1B1 directed to an epitope overlapping the CD4-binding site (2) strongly inhibited sCD4 binding, 2G12 had very little effect.

Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.