Human Anti-High mannose glycans Recombinant Antibody (clone 2G12)
CAT#: HPAB-S0061-YC
Human monoclonal antibody 2G12 efficiently neutralizes a broad range of HIV-1 primary isolates in vitro and protects from in vivo viral challenge in macaques in combination with other antibodies. MAb 2G12 binds with high affinity to a unique, conserved epitope on the HIV-1 envelope that is formed by a cluster of N-linked, high-mannose glycan groups on the "silent" face of gp120.
Published Data
Figure 1 Deposition of complement factor C3 on CEM. NKR cells infected with HIV-1MN.
(A) Control staining with active complement in the absence of antibody; (B) MAb 2G12 with active complement; (C) MAb 3D6 with active complement (negative control); (D) HIV1 hIgG with active complement.
Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.
Figure 2 ADCC mediated by MAb 2G12.
MAb 2G12 is a potent mediator of ADCC as it induces specific lysis of three of the tested targets (gp120/IIIB, IIIB, and MN) at concentrations of , 1 μg/ml.
Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.
Figure 3 Effect of sCD4 on 2G12 and 1B1 binding to gp120.
MAbs 2G12 (●) and 1B1 (▲) were bound to gp160 coated onto ELISA plates, and then incubation with sCD4 and detection of bound sCD4 by the murine anti-CD4 MAb OKT4 were performed. The mean binding value (mean of four replicate wells) obtained with sCD4 in the absence of MAb is represented by the dashed line. While human MAb 1B1 directed to an epitope overlapping the CD4-binding site (2) strongly inhibited sCD4 binding, 2G12 had very little effect.
Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., ... & Katinger, H. (1996). Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of virology, 70(2), 1100-1108.
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- HIV-1
- Species Reactivity
- HIV-1
- Clone
- 2G12
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- High-mannose glycans
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for HPAB-S0061-YC. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 2G12"
- CAT
- Product Name
See other products for "High mannose glycans"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0061-YC-F(E) | Human Anti-High mannose glycans Recombinant Antibody (clone 2G12); Fab Fragment | ELISA, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0061-YC-S(P) | Human Anti-High mannose glycans Recombinant Antibody (clone 2G12); scFv Fragment | ELISA, Neut | Human scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application:
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, FC, WB, Inhib, IHC
Application: ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
