Human Anti-HIV-1 gp120 Recombinant Antibody (clone MAb 2158) (CAT#: HPAB-S0052-YC)
This is a anti-gp120 monoclonal antibody (MAb) 2158, which targets a conformational V2 epitope overlapping the 47 integrin binding site, and constructed a full-length model of V1V2. MAb 2158 was isolated from a subtype B virusinfected subject and is broadly cross-reactive and capable of neutralizing many tier 1 viruses. However, it does not neutralize tier 2 viruses, even though it can bind quite a few tier 2 gp120s; an increased length of V2 and increased total number of glycosylation sites within the hypervariable region are possibilities for the discordance between 2158 binding and neutralization.
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Figure 1 Representative neutralization curves for MAbs against SF162 and JR-FL parental and JR(SF-V1/V2) and SF(JR-V1/V2) Env pseudotypes.
The preferential neutralization of the SF162 Env seen with most of the MAbs tested also correlated with the origin of the V1/V2 domain.
Pinter, A., Honnen, W. J., He, Y., Gorny, M. K., Zolla-Pazner, S., & Kayman, S. C. (2004). The V1/V2 domain of gp120 is a global regulator of the sensitivity of primary human immunodeficiency virus type 1 isolates to neutralization by antibodies commonly induced upon infection. Journal of virology, 78(10), 5205-5215.
Figure 2 MAb 2158 binding of V2 mutants.
Point mutations were introduced into the 1FD6 scaffolded V1V2 (CAP45 sequence) and tested for binding with MAb 2158. Mutants were made to interrogate positions in the strand B-C region (residues 160 to 171) (A), the strand C tail region (residues 172 to 175) (B), the integrin binding site (residues 179 to 181) (C), and the variable sequence region (residues 183 to 193) (D). The ELISA result for each mutation is shown as a percentage of binding relative to that of the wild type (WT).
Spurrier, B., Sampson, J., Gorny, M. K., Zolla-Pazner, S., & Kong, X. P. (2014). Functional implications of the binding mode of a human conformation-dependent V2 monoclonal antibody against HIV. Journal of virology, 88(8), 4100-4112.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- HIV-1
- Species Reactivity
- HIV-1
- Clone
- MAb 2158
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
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