Recombinant mouse antibody to Pol. mAb33 binds to the C-term domain of Integrase and inhibits strongly IN activity.
Figure 1 ELISA data for CTD substituted HIV-1 sIN proteins and their interactions with mAb33.
A high binding microtiter plate was coated with 50 ng of antigen (sIN or one of the Ala substituted sIN proteins) and incubated overnight at 4°C.
Ramcharan, J., Colleluori, D. M., Merkel, G., Andrake, M. D., & Skalka, A. M. (2006). Mode of inhibition of HIV-1 Integrase by a C-terminal domain-specific monoclonal antibody. Retrovirology, 3(1), 34.
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