Mouse Anti-HIV-1 RT Recombinant Antibody (clone 7C4); Fab Fragment (CAT#: FAMAB-0297JF-F(E))

Figure 1 Mouse Anti-HIV-1 RT Recombinant Antibody Fab Fragment (FAMAB-0297JF-F(E)) in SDS-PAGE

SDS-PAGE analysis of FAMAB-0297JF-F(E) in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (heavy chain and light chain) migrate as about 25 kDa.

Figure 2 Mouse Anti-HIV-1 RT Recombinant Antibody Fab Fragment (FAMAB-0297JF-F(E)) in SEC-HPLC

The purity of FAMAB-0297JF-F(E) was 99.3664% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Mouse Anti-HIV-1 RT Recombinant Antibody Fab Fragment (FAMAB-0297JF-F(E)) in WB

Western blot analysis of FAMAB-0297JF-F(E) was performed by loading recombinant reverse transcriptase onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0297JF-F(E) and HRP conjugated Anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (1.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (0.5 μg)

Figure 4 Mouse Anti-HIV-1 RT Recombinant Antibody Fab Fragment (FAMAB-0297JF-F(E)) in ELISA

ELISA analysis of FAMAB-0297JF-F(E) was performed by coating with recombinant reverse transcriptase. Then blocked with BSA and incubated with FAMAB-0297JF-F(E). The HRP conjugated Anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.