Mouse Anti-HK1 Monoclonal Antibody (clone 7A7) (CAT#: VS7-0425-WR63)
This product is a mouse IgG1 antibody that binds to human HK1. The antibody 7A7 can be used for WB, IHC, and ICC assays. It cross reacts with rat and mouse.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/946_E10_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/946_E10_2_selected.jpg
Figure 3 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7043/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7043/ihc_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/ihc_selected.jpg
Figure 5 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/ihc_selected.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_6_6.jpg
Figure 7 Colon
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_9_3.jpg
Figure 8 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_8_4.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156515-HK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156515-HK1
Specifications
- Immunogen
- Recombinant human HK1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HK1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 7A7
- Molecule Class
- Enzyme
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated
- Related Disease
- Anemia, congenital, non-spherocytic hemolytic
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Enzyme-linked Immunosorbent Assay: 1:10000
Immunocytochemistry: 1:200 - 1:1000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Western Blot: 1:500 - 1:2000
Target
- Alternative Names
- HK; HKD; HKI; HXK1; NMSR; RP79; HMSNR; CNSHA5; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
- Gene ID
- 3098
- UniProt ID
- P19367
- Long Name
- Hexokinase 1
- Cellular Localization
- Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000179.2
- Function
- Allosteric enzyme; Kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1614 | Rabbit Anti-HK1 Recombinant Antibody (clone DS1614AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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