Mouse Anti-HK2 Recombinant Antibody (VS3-CJ1032)
CAT#: VS3-CJ1032
This product is a mouse antibody that recognizes human HK2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/1912_G4_5_cr5c862f4c55d36_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61711/1101_E6_1_selected.jpg
Normal Tissue
Figure 3 Epididymis
High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_1_6_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_B_8_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159399-HK2
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Specifications
- Immunogen
- Recombinant protein between 1-170 amino acids from human HK2 (Hexokinase II)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HK2
- Species Reactivity
- Human
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry-Parraffin (1:10-1:50).
Target
- Alternative Names
- HKII; HXK2
- Gene ID
- 3099
- UniProt ID
- P52789
- Sequence Similarities
- Belongs to the hexokinase family.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane
- Protein Refseq
- NP_000180.2
- Function
- Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880).
Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880).
Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1615 | Rabbit Anti-HK2 Recombinant Antibody (clone DS1615AB) | WB, IHC-P, IF | Rabbit IgG |
| MOR-4588 | Rabbit Anti-HK2 Recombinant Antibody (clone TH101DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0618-WJ | Mouse Anti-HK2 Recombinant Antibody (HPAB-0618-WJ) | IF | Mouse IgG |
| ZG-0420J | Mouse Anti-HK2 Recombinant Antibody (ZG-0420J) | WB | Mouse IgG |
| ZG-0421J | Mouse Anti-HK2 Recombinant Antibody (ZG-0421J) | WB | Mouse IgG |
| VS3-XY808 | Mouse Anti-HK2 Recombinant Antibody (clone 3D3) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-FY2736 | Mouse Anti-HK2 Recombinant Antibody (VS3-FY2736) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0618-WJ-F(E) | Mouse Anti-HK2 Recombinant Antibody; Fab Fragment (HPAB-0618-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0618-WJ-S(P) | Mouse Anti-HK2 Recombinant Antibody; scFv Fragment (HPAB-0618-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY249 | Rabbit Anti-NHP HK2 Recombinant Antibody (VS-1024-XY249) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1027 | Anti-HK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3162 | Anti-Human HK2 Immunohistochemistry Kit | IHC |
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