Mouse Anti-HSP70 Recombinant Antibody (clone 3G10)
CAT#: MOB-0046F
This product is a mouse antibody that recognizes HSP70.
Tested Data
IF
Figure 1 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in IF
Immunofluorescence analysis of Hela cells. 1. AIM2 polyclonal antibody (red) was diluted at 1:200 (overnight at 4°C). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 594 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes).
WB
Figure 2 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in WB
Western blot analysis of HT-29, NIH/3T3 and HepG2 cell lysates, the primary antibody was diluted 1:1000, overnight at 4°C, and the second antibody (diluted 1:10000, 37°C for 1 hour.
IHC
Figure 3 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in IHC
Immunohistochemical analysis of paraffin-embedded human-uterine cancer tissue. 1. HSP70 monoclonal antibody (3G10) is diluted at 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
IHC
Figure 4 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1. HSP70 monoclonal antibody (3G10) was diluted at 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. The secondary antibody is diluted 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
IF
Figure 5 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in IF
Immunofluorescence analysis of human breast cancer tissue. 1. HSP70 monoclonal antibody (3G10) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
WB
Figure 6 Mouse Anti-HSP70 Recombinant Antibody (clone 3G10) (MOB-0046F) in WB
1) Hela, 2) Western blot analysis of mouse brain, diluted 1:2000.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSP70
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3G10
- Applications
- WB, IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
- Alternative Names
- Heat shock protein 70; HSP70
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| VS3-FY701 | Recombinant Mouse Anti-HSPA4 Antibody (clone 2E4F10) | ELISA, WB, IHC, FC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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