Mouse Anti-HSPA1A Recombinant Antibody (clone 2A11-F3-G12)
CAT#: VS3-QX552
This product is a mouse antibody that recognizes HSPA1A. The antibody can be used in applications: WB, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in the basal layer of glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_1_5_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204389-HSPA1A
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Specifications
- Immunogen
- Purified recombinant human Hsp70(C-terminus) protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Hsp70(C-term)
- Species Reactivity
- Human
- Clone
- 2A11-F3-G12
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.1M Tris-Glycine (pH 7.4,150 mM NaCl), 0.1mg/ml BSA and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-517-F(E) | Recombinant Anti-human HSPA1A Antibody Fab Fragment | WB, RIA, FuncS | Fab |
| MHH-517-F(E) | Recombinant Human Anti-human HSPA1A Antibody Fab Fragment | WB, FC, FuncS | Fab |
| HPAB-0243-YC-F(E) | Mouse Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0243-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0244-YC-F(E) | Human Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0244-YC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-517-S(P) | Recombinant Anti-human HSPA1A Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-517-S(P) | Recombinant Human Anti-human HSPA1A Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| HPAB-0243-YC-S(P) | Mouse Anti-HSPA1A Recombinant Antibody scFv Fragment (HPAB-0243-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0244-YC-S(P) | Human Anti-HSPA1A Recombinant Antibody scFv Fragment (HPAB-0244-YC-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-916CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 2A4) | Block, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-917CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 3A3) | Block, ELISA, FC, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG1 |
| NEUT-918CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 4G4) | Inhib, IHC-P, ICC, IF, FC, WB | Mouse IgG1 |
| NEUT-919CQ | Mouse Anti-HSPA1A Recombinant Antibody (clone 5A5) | Block, FC, GSA, ICC, IF, IHC, IHC-Fr, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1692 | Rabbit Anti-HSPA1A Recombinant Antibody (clone DS1692AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY132 | AbPlus™ Anti-HSPA1A Magnetic Beads (2A4) | IP, Protein Purification | |
| VS-0724-YC1220 | AbPlus™ Anti-HSPA1A Magnetic Beads (VS-0724-YC1220) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY198 | Mouse Anti-HSPA1A scFv-Fc Chimera (VS-0325-FY198) | ELISA, FC | Mouse IgG1, scFv-Fc |
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