Chicken Anti-HSPB3 Recombinant Antibody (clone 7B4) (CAT#: FAMAB-0003JF)
Recombinant chicken monoclonal antibody specially binds to human HspB3. This anti-HspB3 antibody 7B4 can be potentially wildly used in the treatment of motor neuropathy.
Specific Inquiry
WB
Figure 1 Western blot analysis showing the tissue specificity of the expression of each sHSP.
The newly raised anti-HSPB3 antibody specifically detects a 17-kDa protein in human skeletal muscle extracts.
Sugiyama, Y., Suzuki, A., Kishikawa, M., Akutsu, R., Hirose, T., Waye, M. M., ... & Ohno, S. (2000). Muscle develops a specific form of small heat shock protein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation. Journal of Biological Chemistry, 275(2), 1095-1104.
IHC
Figure 2 HSPB3 is localized on myotube-specific actin bundles.
Original magnification × 25.
Sugiyama, Y., Suzuki, A., Kishikawa, M., Akutsu, R., Hirose, T., Waye, M. M., ... & Ohno, S. (2000). Muscle develops a specific form of small heat shock protein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation. Journal of Biological Chemistry, 275(2), 1095-1104.
IF
Figure 3 Immunofluorescencelabeling of human dermiswith antibodies to candidatefibroblast subpopulation markersidentified by spatial transcriptomics.
Expression of HSPB3 is enrichedin the papillary dermis (HSPB3,green).
Philippeos, C., Telerman, S. B., Oulès, B., Pisco, A. O., Shaw, T. J., Elgueta, R., ... & Watt, F. M. (2018). Spatial and single-cell transcriptional profiling identifies functionally distinct human dermal fibroblast subpopulations. Journal of Investigative Dermatology, 138(4), 811-825.
FC
Figure 4 Human dermal fibroblast subpopulations maintain functional differences in vitro.
Data are representative of at least 3 independent experiments.
Philippeos, C., Telerman, S. B., Oulès, B., Pisco, A. O., Shaw, T. J., Elgueta, R., ... & Watt, F. M. (2018). Spatial and single-cell transcriptional profiling identifies functionally distinct human dermal fibroblast subpopulations. Journal of Investigative Dermatology, 138(4), 811-825.
Specifications
- Immunogen
- Antigen mixture 1 (annexin XI, centromere protein B, DNA topoisomerase I, histidyl tRNA synthetase, Ro60, Rpp38 and U1A) and mixture 2 (a-fodrin, human Pop1 protein, heat shock protein B3, Ro52, Rpp30, Rrp4 and Rrp42)
- Host Species
- Chicken
- Derivation
- Chicken phage display scFv library
- Specificity
- Human HspB3
- Species Reactivity
- Human
- Clone
- 7B4
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Motor neuropathy
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HSPB3 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0003JF-F(E) | Chicken Anti-HSPB3 Recombinant Antibody (clone 7B4); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0003JF-S(P) | Chicken Anti-HSPB3 Recombinant Antibody (clone 7B4); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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