Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (CAT#: PABJ-0152)

This product is a Human antibody that recognizes IAV H3N2 HA.

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SDS-PAGE

Figure 1 Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (PABJ-0152) in SDS-PAGE

Figure 1 Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (PABJ-0152) in SDS-PAGE

SDS-PAGE analysis of PABJ-0152 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

SEC-HPLC

Figure 2 Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (PABJ-0152) in SEC-HPLC

Figure 2 Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (PABJ-0152) in SEC-HPLC

HPLC analysis of PABJ-0152 shows the purity is>95%.

WB

Figure 3 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in WB

Figure 3 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in WB

Western blot analysis of PABJ-0152 was performed with Recombinant Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin Protein (HA1 Subunit) (His Tag).
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Human IgG (H+L)

ELISA

Figure 4 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in ELISA

Figure 4 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in ELISA

ELISA analysis of PABJ-0152 was performed by coating with Recombinant Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin Protein (HA1 Subunit) (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)

DB

Figure 5 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in Dot Blot

Figure 5 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in Dot Blot

Dot Blot analysis of PABJ-0152 was performed with Recombinant Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin Protein (HA1 Subunit) (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • IAV H3N2 HA
  • Species Reactivity
  • IAV H3N2
  • Clone
  • S5V2-29
  • Applications
  • ELISA, Neut, FuncS

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Functional Assay.

Target

  • Alternative Names
  • Influenza A virus Hemagglutinin; Influenza A virus; Hemagglutinin; HA; IAV; H3; H3N2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABJ-0152, RRID: AB_3111609)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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