Anti-ID1 Antibody Prodrug, Protease Activated (#BCH-2/#5-3)
CAT#: VS-1025-YC154
The Anti-ID1 Antibody Prodrug, Protease Activated (#BCH-2/#5-3) is an antibody prodrug comprised of a recombinant rabbit antibody targeting human ID1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to ID1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to ID1 effectively. ID1 (Inhibitor of Differentiation 1) is a helix-loop-helix protein that acts as a potent oncogene by inhibiting the differentiation of tumor cells and promoting an aggressive, stem-like phenotype. Its high expression is associated with poor prognosis and enhanced metastasis in a variety of solid tumors.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HaCaT, Hep G2, PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
❮
❯
Specifications
- Host Animal
- Rabbit
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human ID1
- Target
- ID1
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- #BCH-2/#5-3
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- ID; bHLHb24
- Gene ID
- 3397
- UniProt ID
- P41134
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone #BCH-2/#5-3"
- CAT
- Product Name
See other products for "ID1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3006z | Mouse Anti-ID1 Recombinant Antibody (clone 29D9) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2314MZ | Recombinant Mouse Anti-Human ID1 Antibody (clone 3567D2b) | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1729 | Rabbit Anti-Id1 Recombinant Antibody (clone DS1729AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0309-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0309-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0309-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0418-CN | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2) | WB, ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0418-CN-S(P) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); scFv Fragment | WB, ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0418-CN-F(E) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); Fab Fragment | WB, ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1086 | Anti-ID1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3378 | Anti-Mouse ID1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3377 | Anti-Human ID1 Immunohistochemistry Kit | IHC |
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