Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3)
CAT#: HPAB-0307-YC
Provided is a rabbit monoclonal antibody that binds to human Id1 protein with high specificity and high affinity. The antibody has no substantial cross-reactivity with other family Id proteins such as Id2, Id3, or Id4, or other endogenous proteins present in the cells that express Id1 protein. The high specificity and high affinity of the rabbit monoclonal antibodies of the present invention allows sensitive and specific detection and/or quantitation of Id1protein in biological samples.
Published Data
Gene Expression
ELISA
Figure 1 Calibration curves obtained following addition of known amounts of human Id1: His to buffered bovine serum albumin and analyzing the resulting solutions using rabbit anti-human Id1 in a standard ELISA format.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HaCaT, Hep G2, PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
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Specifications
- Immunogen
- Human Id1:KLH fusion protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ID1
- Species Reactivity
- Human
- Clone
- #BCH-2/#5-3
- Applications
- ELISA, WB, IHC
- Related Disease
- Breast cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone #BCH-2/#5-3"
- CAT
- Product Name
See other products for "ID1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3006z | Mouse Anti-ID1 Recombinant Antibody (clone 29D9) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MOB-2314MZ | Recombinant Mouse Anti-Human ID1 Antibody (clone 3567D2b) | IHC-P, WB | Mouse antibody |
| HPAB-0418-CN | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2) | WB, ELISA, IHC | Rabbit IgG |
| MRO-0808-CN | Rabbit Anti-ID1 Recombinant Antibody (clone JM92-13) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-XY3390 | Rabbit Anti-ID1 Recombinant Antibody (clone R01-2A4) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1729 | Rabbit Anti-Id1 Recombinant Antibody (clone DS1729AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0309-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0418-CN-S(P) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); scFv Fragment | WB, ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0309-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0418-CN-F(E) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); Fab Fragment | WB, ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1086 | Anti-ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3378 | Anti-Mouse ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3377 | Anti-Human ID1 Immunohistochemistry Kit | IHC |
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