Human Anti-IDH2 Recombinant Antibody (clone 2Q1)
CAT#: VS-0724-YC13
The antibody targets the tumor-specific isocitrate dehydrogenase 2 (IDH2) presented on the cell surface in complex with a common human leukocyte antigen allele, HLA-B*07:02. The specificity of clone 2Q1 was further assessed by surface plasmon resonance (SPR). At high concentrations, there was detectable binding of clone 2Q1 to IDH2WT-HLA-B*07:02 with a calculated KD> 800 nM. In contrast, clone 2Q1 bound more tightly to IDH2R140Q-HLA-B*07:02 with a KD of 44 nM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_8_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_6_6.jpg
Normal Tissue
Figure 7 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182054-IDH2
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human dehydrogenase 2 (IDH2)
- Species Reactivity
- Human
- Clone
- 2Q1
- Applications
- ELISA, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IDH; IDP; IDHM; IDPM; ICD-M; IDH-2; D2HGA2; mNADP-IDH
- Gene ID
- 3418
- UniProt ID
- Q53GL5
- Long Name
- dehydrogenase 2 (IDH2)
- Sequence Similarities
- Belongs to the isocitrate and isopropylmalate dehydrogenases family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation at Lys-413 dramatically reduces catalytic activity.
Deacetylated by SIRT3.
Ubiquitination at Lys166 and Lys272
- Protein Refseq
- NP_001276839.1; NP_001277043.1; NP_002159.2
- Function
- Plays a role in intermediary metabolism and energy production.
It may tightly associate or interact with the pyruvate dehydrogenase complex.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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