Human Anti-IDH2 Recombinant Antibody (clone 2Q1) (CAT#: VS-0724-YC13)
The antibody targets the tumor-specific isocitrate dehydrogenase 2 (IDH2) presented on the cell surface in complex with a common human leukocyte antigen allele, HLA-B*07:02. The specificity of clone 2Q1 was further assessed by surface plasmon resonance (SPR). At high concentrations, there was detectable binding of clone 2Q1 to IDH2WT-HLA-B*07:02 with a calculated KD> 800 nM. In contrast, clone 2Q1 bound more tightly to IDH2R140Q-HLA-B*07:02 with a KD of 44 nM.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/if_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_8_8.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_5.jpg
Figure 6 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_6_6.jpg
Figure 7 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_4_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_4_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182054-IDH2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182054-IDH2
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human dehydrogenase 2 (IDH2)
- Species Reactivity
- Human
- Clone
- 2Q1
- Applications
- ELISA, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IDH; IDP; IDHM; IDPM; ICD-M; IDH-2; D2HGA2; mNADP-IDH
- Gene ID
- 3418
- UniProt ID
- Q53GL5
- Long Name
- dehydrogenase 2 (IDH2)
- Sequence Similarities
- Belongs to the isocitrate and isopropylmalate dehydrogenases family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation at Lys-413 dramatically reduces catalytic activity.
Deacetylated by SIRT3.
Ubiquitination at Lys166 and Lys272
- Protein Refseq
- NP_001276839.1; NP_001277043.1; NP_002159.2
- Function
- Plays a role in intermediary metabolism and energy production.
It may tightly associate or interact with the pyruvate dehydrogenase complex.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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