Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0) (CAT#: VS3-FY723)

Name: Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0)
SKU: VS3-FY723
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes IDH2. This antibody has been reported for use in Western Blot. The clone R04-1B0 is specific for human, rat IDH2. The antigen is synthetic peptide of human idh2.

Custom Production

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Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0) in WB.

Figure 1. Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0) in WB.

Western Blot analysis of Jurkat, IDH2 in rat brain lysates using IDH2 Antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/if_selected.jpg

Normal Tissue

Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_8_8.jpg

Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_7_5.jpg

Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_3.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_5.jpg

Figure 6 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_6_6.jpg

Figure 7 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_4_6.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182054-IDH2

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human IDH2.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Rat IDH2

Species Reactivity
Human, Rat

Clone
R04-1B0

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 51 kDa; Observed MW: 51 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial

Gene ID
3418

UniProt ID
P48735

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IDH2"

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IgG Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-2708z	Mouse Anti-IDH2 Recombinant Antibody (clone 35C2)	WB, ICC, IF, IHC	Mouse IgG1
MOB-2317MZ	Recombinant Mouse Anti-Human IDH2 Antibody	ICC/IF, IHC-P, WB	Mouse antibody
MRO-0810-CN	Rabbit Anti-IDH2 Recombinant Antibody (clone JA55-31)	WB, IHC, FC	Rabbit IgG
VS7-0425-WR114	Mouse Anti-IDH2 Recombinant Antibody (clone 3E8D12)	IHC, FC	Mouse IgG1
VS7-0425-WR115	Mouse Anti-IDH2 Recombinant Antibody (clone 3E8E9)	IHC, FC	Mouse IgG1

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