Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0)
CAT#: VS3-FY723
This product is a recombinant rabbit antibody that recognizes IDH2. This antibody has been reported for use in Western Blot. The clone R04-1B0 is specific for human, rat IDH2. The antigen is synthetic peptide of human idh2.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-IDH2 Antibody (clone R04-1B0) in WB.
Western Blot analysis of Jurkat, IDH2 in rat brain lysates using IDH2 Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_8_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_A_6_6.jpg
Normal Tissue
Figure 7 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7831/21125_B_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182054-IDH2
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Specifications
- Immunogen
- Synthetic peptide of human IDH2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat IDH2
- Species Reactivity
- Human, Rat
- Clone
- R04-1B0
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "IDH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2708z | Mouse Anti-IDH2 Recombinant Antibody (clone 35C2) | WB, ICC, IF, IHC | Mouse IgG1 |
| MOB-2317MZ | Recombinant Mouse Anti-Human IDH2 Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| MRO-0810-CN | Rabbit Anti-IDH2 Recombinant Antibody (clone JA55-31) | WB, IHC, FC | Rabbit IgG |
| VS3-FY722 | Recombinant Rabbit Anti-IDH2 Antibody (clone R01-7E4) | WB, IHC-P | Rabbit IgG |
| VS-0923-FY102 | Recombinant Mouse Anti-IDH2 Antibody (VS-0923-FY102) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1734 | Rabbit Anti-IDH2 Recombinant Antibody (clone DS1734AB) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1088 | Anti-IDH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3386 | Anti-Mouse IDH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3385 | Anti-Human IDH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3387 | Anti-Rat IDH2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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