Rabbit Anti-IFRD1 Recombinant Antibody (clone R05-2H2)
CAT#: VS3-FY1949
This anti-IFRD1 antibody plays a role in western blot analysis of IFRD1 in Ramos lysates, western blot demonstrated that R05-2H2 recognized a target protein with a molecular weight of 50 kDa. This clone R05-2H2 is a rabbit recombinant monoclonal antibody which has a high affinity of human IFRD1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24122/53268_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000006652-IFRD1
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WB
Figure 1 Anti-IFRD1 Antibody (VS3-FY1949) in WB.
The IFRD1 expression in Ramos lysates were detected using the anti-IFRD1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human IFRD1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IFRD1
- Species Reactivity
- Human
- Clone
- R05-2H2
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Cystic fibrosis lung disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Interferon Related Developmental Regulator 1
- Gene ID
- 3475
- UniProt ID
- O00458
- Sequence Similarities
- Belongs to the IFRD family.
- Cellular Localization
- nucleus, sarcoplasm
- Post Translation Modifications
- Ubiquitination at Lys174 and Lys175 (NX_O00458 [NX_O00458-1])
Modification sites at PhosphoSitePlus (O00458)
- Protein Refseq
- NP_001007246.1; NP_001184008.1; NP_001184009.1; NP_001541.2
- Function
- Could play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF. May be an autocrine factor that attenuates or amplifies the initial ligand-induced signal (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IFRD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2324MZ | Recombinant Mouse Anti-Mouse IFRD1 Antibody (clone JSU648) | ELISA, ICC, WB | Mouse antibody |
| VS13-YC568 | Rabbit Anti-IFRD1 Recombinant Antibody (VS13-YC568) | WB | Rabbit IgG |
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