CytoStream™ Rabbit Anti-IGF2BP3 Polyclonal Antibody (VS13-YC572)
CAT#: VS13-YC572
The CytoStream™ Rabbit Anti-IGF2BP3 Polyclonal Antibody is specific for human, mouse and rat IGF2BP3. IGF2BP3 represses IGF II translation, particularly in late development stages, featuring RNA binding domains integral to RNA synthesis processes with chromosomal pseudogenes identified. The anti-IGF2BP3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2037/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76951/168373_A_7_5.jpg
Normal Tissue
Figure 3 Placenta
Cytotrophoblasts Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: Medium Intensity: Strong Quantity: <25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76951/168373_A_1_7.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76951/168373_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HUVEC TERT2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136231-IGF2BP3
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Specifications
- Immunogen
- Recombinant human IMP3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat IGF2BP3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IF, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "IGF2BP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0312MZ | Recombinant Mouse Anti-Human IGF2BP3 Antibody (clone A70.2) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1758 | Rabbit Anti-IGF2BP3 Recombinant Antibody (clone DS1758AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0839-CN | Mouse Anti-IGF2BP3 Recombinant Antibody (clone C2-E4) | WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1975-CN | Rabbit Anti-IGF2BP3 Polyclonal Antibody (MRO-1975-CN) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX583 | Mouse Anti-IGF2BP3 Recombinant Antibody (clone 1F1-E10-D11) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY845 | Mouse Anti-IGF2BP3 Recombinant Antibody (clone 8F11) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1104 | Anti-IGF2BP3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3438 | Anti-Human IGF2BP3 Immunohistochemistry Kit | IHC |
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