Rabbit Anti-IKZF1 Recombinant Antibody (clone R08-5E4) (CAT#: VS3-FY1747)
Clone R08-5E4 is a rabbit recombinant monoclonal antibody that detects human IKZF1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, IHC-P. This Ikaros antibody was used in western blot analysis of Ikaros in CHO-K1, Jurkat lysates.
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WB
Figure 1 Anti-IKZF1 Antibody (VS3-FY1747) in WB.
The Ikaros expression in CHO-K1, Jurkat lysates were detected using the anti-Ikaros by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human icaros
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster IKZF1
- Species Reactivity
- Human, Hamster
- Clone
- R08-5E4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 50-70 kDa.
- Related Disease
- Immunodeficiency, Common Variable, 13 and Diamond-Blackfan Anemia-Like
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- IKAROS Family Zinc Finger 1
- Gene ID
- 10320
- UniProt ID
- Q13422
- Sequence Similarities
- Belongs to the Ikaros C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity).
Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
- Protein Refseq
- XP_011513360.1; XP_011513361.1; XP_011513362.1; XP_011513363.1; XP_011513364.1
- Function
- Transcription regulator of hematopoietic cell differentiation.
Binds gamma-satellite DNA.
Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1783 | Rabbit Anti-IKZF1 Recombinant Antibody (clone DS1783AB) | IHC-P, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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