Rabbit Anti-IKZF1 Recombinant Antibody (clone R08-5E4)
CAT#: VS3-FY1747
Clone R08-5E4 is a rabbit recombinant monoclonal antibody that detects human IKZF1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, IHC-P. This Ikaros antibody was used in western blot analysis of Ikaros in CHO-K1, Jurkat lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/1779_C1_2_selected.jpg
Normal Tissue
Figure 2 High expression in tonsil
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/140975_A_6_8_val_selected.jpg
Normal Tissue
Figure 3 Low expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/140975_B_7_5_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35222/140899_A_8_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35222/140899_A_5_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, JURKAT, MOLT-4, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185811-IKZF1
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WB
Figure 1 Anti-IKZF1 Antibody (VS3-FY1747) in WB.
The Ikaros expression in CHO-K1, Jurkat lysates were detected using the anti-Ikaros by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human icaros
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster IKZF1
- Species Reactivity
- Human, Hamster
- Clone
- R08-5E4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 50-70 kDa.
- Related Disease
- Immunodeficiency, Common Variable, 13 and Diamond-Blackfan Anemia-Like
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- IKAROS Family Zinc Finger 1
- Gene ID
- 10320
- UniProt ID
- Q13422
- Sequence Similarities
- Belongs to the Ikaros C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity).
Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
- Protein Refseq
- XP_011513360.1; XP_011513361.1; XP_011513362.1; XP_011513363.1; XP_011513364.1
- Function
- Transcription regulator of hematopoietic cell differentiation.
Binds gamma-satellite DNA.
Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IKZF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1783 | Rabbit Anti-IKZF1 Recombinant Antibody (clone DS1783AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0484J | Mouse Anti-IKZF1 Recombinant Antibody (ZG-0484J) | WB | Mouse IgG |
| ZG-0485J | Mouse Anti-IKZF1 Recombinant Antibody (ZG-0485J) | WB | Mouse IgG |
| VS3-QX590 | Mouse Anti-IKZF1 Recombinant Antibody (clone 1A12-F2-D8) | WB, IP | Mouse IgG1 |
| VS7-0425-WR128 | Mouse Anti-IKZF1 Recombinant Antibody (clone 1C1B6) | ICC, FC | Mouse IgG2b |
| VS7-0425-WR129 | Mouse Anti-IKZF1 Recombinant Antibody (clone 4B6A12) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3472 | Anti-IKZF1 Immunohistochemistry Kit | IHC |
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