Mouse Anti-IKZF1 Recombinant Antibody (clone 0725W21)
CAT#: VS-0725-FY178
This recombinant antibody, clone 0725W21, targets the N-terminal half of mouse Ikaros. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), and Western Blot (WB). IKZF1 is a zinc-finger DNA-binding transcription factor involved in chromatin remodeling and lymphocyte differentiation. It acts as a regulator of B- and T-cell development. This antibody is a valuable tool for studying gene regulation in hematopoiesis and its implications in lymphoid malignancies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/1779_C1_2_selected.jpg
Normal Tissue
Figure 2 High expression in tonsil
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/140975_A_6_8_val_selected.jpg
Normal Tissue
Figure 3 Low expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35221/140975_B_7_5_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35222/140899_A_8_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35222/140899_A_5_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, JURKAT, MOLT-4, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185811-IKZF1
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Specifications
- Immunogen
- A recombinant protein representing the N-terminal half of mouse Ikaros.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse IKZF1
- Species Reactivity
- Mouse
- Clone
- 0725W21
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Western Blot
- Related Disease
- Acute lymphoblastic leukemia, Common variable immunodeficiency
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 62 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 4 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 4 µg each
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- IKAROS Family Zinc Finger 1
- Gene ID
- 22778
- UniProt ID
- Q03267
- Long Name
- IKAROS Family Zinc Finger 1
- Sequence Similarities
- Belongs to the Ikaros C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity).
Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
- Protein Refseq
- XP_011513360.1; XP_011513361.1; XP_011513362.1; XP_011513363.1; XP_011513364.1
- Function
- Transcription regulator of hematopoietic cell differentiation.
Binds gamma-satellite DNA.
Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "IKZF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4031z | Mouse Anti-IKZF1 Recombinant Antibody (clone 20G3) | WB, FC, ICC, IP | Mouse IgG1 |
| MRO-0821-CN | Rabbit Anti-IKZF1 Recombinant Antibody (clone JB50-38) | WB, IHC, FC | Rabbit IgG |
| VS3-QX590 | Mouse Anti-IKZF1 Recombinant Antibody (clone 1A12-F2-D8) | WB, IP | Mouse IgG1 |
| VS3-FY1747 | Rabbit Anti-IKZF1 Recombinant Antibody (clone R08-5E4) | WB, IHC-P | Rabbit IgG |
| VS3-FY2742 | Mouse Anti-IKZF1 Recombinant Antibody (VS3-FY2742) | FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1783 | Rabbit Anti-IKZF1 Recombinant Antibody (clone DS1783AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3472 | Anti-IKZF1 Immunohistochemistry Kit | IHC |
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