Rabbit Anti-INPP5D Recombinant Antibody (clone 9G11)
CAT#: ZG-0768J
This product is a rabbit antibody that recognizes INPP5D.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-INPP5 Antibody (ZG-0768J) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate
All lanes: INPP5D antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 134, 110 kDa
Observed band size: 145 kDa
IHC
Figure 2 Rabbit Anti-INPP5 Antibody (ZG-0768J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 3 Rabbit Anti-INPP5 Antibody (ZG-0768J) in IP
Immunoprecipitating SHIP in Raji whole cell lysate
Lane 1: Rabbit control IgG in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0768J (2µg)+ Raji whole cell lysate (500µg)
Lane 3: Raji whole cell lysate (10µg)
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70455/1410_B8_2_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70455/157563_A_5_8_val_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16300/37086_B_5_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16300/37086_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16300/37086_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16300/37086_A_8_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, HEL, HMC-1, hTCEpi, NB-4, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168918-INPP5D
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Specifications
- Immunogen
- A synthesized peptide derived from human SHIP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human INPP5D
- Species Reactivity
- Human
- Clone
- 9G11
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
- Related Disease
- Inflammatory Bowel Disease types such as Crohn's Disease and Ulcerative Colitis
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SH2 domain-containing inositol 5'-phosphatase 1; inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; phosphatidylinositol 4,5-bisphosphate 5-phosphatase; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II
- Gene ID
- 3635
- UniProt ID
- Q92835
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "INPP5D"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1522z | Mouse Anti-INPP5D Recombinant Antibody (clone 38D4) | WB, FC, IHC | Mouse IgG2a |
| MOB-1366CT | Recombinant Mouse anti-Human INPP5D Monoclonal antibody (TIJQ-02) | ELISA, WB | |
| MRO-1388-CN | Rabbit Anti-INPP5D Recombinant Antibody (clone CBACN-500) | WB, IHC, IP, FC | Rabbit IgG |
| MRO-2374-CN | Rabbit Anti-INPP5D Recombinant Antibody (clone CBACN-660) | WB, IF, IP | Rabbit IgG |
| VS3-WK2153 | Rabbit Anti-INPP5D (pY1020) Recombinant Antibody (clone R07-8Z2) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1839 | Rabbit Anti-INPP5D Recombinant Antibody (clone DS1839AB) | ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY154 | AbPlus™ Anti-INPP5D Magnetic Beads (CBACN-500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3565 | Anti-INPP5D Immunohistochemistry Kit | IHC |
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