Rabbit Anti-IRF3 Recombinant Antibody (clone 4H10)
CAT#: ZG-0771J
This product is a rabbit antibody that recognizes IRF3.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-IRF3 Antibody (ZG-0771J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not)
All lanes Phospho-IRF3 antibody at 1.03 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 KDa
Observed band size: 47 KDa
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/15913_A_1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32525_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126456-IRF3
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-IRF3 (S386)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRF3
- Species Reactivity
- Human
- Clone
- 4H10
- Applications
- ELISA, WB
- Conjugate
- Uconjugated
- Related Disease
- Encephalopathy, acute, infection-induced, herpes-specific, 7
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000
Target
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Related Signaling Pathways
TLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "IRF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1855 | Hi-Affi™ Recombinant Rabbit Anti-IRF3 Monoclonal Antibody (DS1855AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0869-CN | Recombinant Rabbit Anti-IRF3 Monoclonal Antibody (CBACN-321) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2340-CN | Recombinant Rabbit Anti-IRF3 (phosphorylated Ser386) Monoclonal Antibody (CBACN-631) | WB, IF | Rabbit IgG |
| VS3-WK962 | Mouse Anti-IRF3 Recombinant Antibody (VS3-WK962) | ICC, IHC, FC | Mouse IgG |
| VS3-WK964 | Rabbit Anti-IRF3 Recombinant Antibody (VS3-WK964) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-WK1587 | Mouse Anti-IRF3 Recombinant Antibody (clone 5H10) | IHC-P, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY285 | Mouse Anti-NHP IRF3 Recombinant Antibody (clone 1522CT766.58.24) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1145 | Anti-IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3587 | Anti-Mouse IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3586 | Anti-Human IRF3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC595 | CytoStream™ Rabbit Anti-IRF3 Recombinant Antibody (VS13-YC595) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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