Mouse Anti-NHP IRF3 Recombinant Antibody (clone 1522CT766.58.24)
CAT#: VS-1024-XY285
Designed for the precise detection of IRF3, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. The antibody was generated using an IRF3 recombinant protein as the immunogen, ensuring targeted specificity. Validated for use in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody is an indispensable tool for in-depth investigation of IRF3 expression and function in a range of experimental applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/15913_A_1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32525_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126456-IRF3
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Specifications
- Immunogen
- IRF3 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- IRF3
- Species Reactivity
- Human, Non-human primate
- Clone
- 1522CT766.58.24
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:20, IHC: 1:25, IF: 1:25, FC: 1:25
Target
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Related Signaling Pathways
TLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1855 | Hi-Affi™ Recombinant Rabbit Anti-IRF3 Monoclonal Antibody (DS1855AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2340-CN | Recombinant Rabbit Anti-IRF3 (phosphorylated Ser386) Monoclonal Antibody (CBACN-631) | WB, IF | Rabbit IgG |
| VS3-WK962 | Mouse Anti-IRF3 Recombinant Antibody (VS3-WK962) | ICC, IHC, FC | Mouse IgG |
| VS3-WK964 | Rabbit Anti-IRF3 Recombinant Antibody (VS3-WK964) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY763 | Recombinant Mouse Anti-IRF3 Antibody (clone 2A10A8) | ELISA, IHC, ICC, FC | Mouse IgG2b |
| VS3-FY764 | Recombinant Mouse Anti-IRF3 Antibody (clone 5G3E2) | ELISA, WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1145 | Anti-IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3587 | Anti-Mouse IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3586 | Anti-Human IRF3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC595 | CytoStream™ Rabbit Anti-IRF3 Recombinant Antibody (VS13-YC595) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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