CytoStream™ Rabbit Anti-IRS2 Recombinant Antibody (VS13-YC597)
CAT#: VS13-YC597
The CytoStream™ Rabbit Anti-IRS2 Recombinant Antibody is specific for human, mouse and rat IRS2. IRS2, encoding the insulin receptor substrate 2, acts as a cytoplasmic signaling molecule in insulin and cytokine pathways. It serves as an adaptor for receptor tyrosine kinases and multiple downstream effectors, with its product phosphorylated upon insulin receptor stimulation and in response to IL4 treatment by an interleukin 4 receptor-associated kinase. The anti-IRS2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54664/881_A4_1_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16944/36406_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16944/36406_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16944/36406_A_6_6.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16944/36406_B_2_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-HME1, LHCN-M2, SH-SY5Y, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185950-IRS2
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Specifications
- Immunogen
- Recombinant human IRS2, aa 1319-1333.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat IRS2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Related Signaling Pathways
Insulin Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "IRS2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3735z | Mouse Anti-IRS2 Recombinant Antibody (clone 19B2) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1862 | Hi-Affi™ Recombinant Rabbit Anti-IRS2 Monoclonal Antibody (DS1862AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3600 | Anti-IRS2 Immunohistochemistry Kit | IHC |
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