Recombinant Mouse Anti-JEV Antibody (2F2) (CAT#: PABC-364)
This product is a recombinant Mouse antibody that can recognize JEV.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 2 Immunofluorescence analysis of S2/ZF-ZR and S2/JEF-JER cells with antibody 2F2.
S2 cells were transfected with pAc/ZF-ZR (A) or pAc/JEF-JER ( B). Transfected cells were harvested in PBS and plated onto microscopic slides. The cells were fixed in 10% formalin with 1% Triton X-100 at room temperature for 30 min. The cells were incubated subsequently with JEV E specific monoclonal antibody 2F2 and a fluorescein-conjugated secondary antibody (DAKO) at room temperature for 1 h. Each incubation was followed by two washes in PBS. Finally the slides were mounted with VECTASHIELD Mounting Medium containing propidium iodide and viewed under an Olympus fluorescence microscope.
Zhang, F., Ma, W., Zhang, L., Aasa-Chapman, M., & Zhang, H. (2007). Expression of particulate-form of Japanese encephalitis virus envelope protein in a stably transfected Drosophila cell line. Virology journal, 4(1), 17.
WB
Figure 3 Comparison of recombinant JEV E protein expressed in deferent cells by Western blot.
Cell lysate were resolved by 12% SDS-PAGE under non-reducing conditions. After electrophoresis proteins were transferred onto nitrocellulose membrane and probed with the antibody 2F2. ECL reagents were used to visualise protein bands. Lanes 1 to 6 are the cell lysate from Vero/ZF-ZR, Vero/JEF-JER, S2/ZF-ZR, S2/JEF-JER, Vero and S2 cells respectively.
Zhang, F., Ma, W., Zhang, L., Aasa-Chapman, M., & Zhang, H. (2007). Expression of particulate-form of Japanese encephalitis virus envelope protein in a stably transfected Drosophila cell line. Virology journal, 4(1), 17.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- JEV
- Species Reactivity
- JEV
- Clone
- 2F2
- Applications
- Neut, IF, WB, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody 2F2 has been reported in applications of Neutralization, Immunofluorescence, Western Blot and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Japanese encephalitis virus; JEV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Mouse Antibody Products
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Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0158MZ | Recombinant Mouse Anti-Japanese Encephalitis Antibody (clone C926M) | ELISA, IF | Mouse antibody |
| PABC-365 | Recombinant Mouse Anti-JEV Antibody (2H4) | WB, Neut, ELISA | Mouse IgG |
| HPAB-1276-FY | Human Anti-JEV Recombinant Antibody (HPAB-1276-FY) | ELISA, Neut, WB | Human IgG1 |
| HPAB-1277-FY | Human Anti-JEV Recombinant Antibody (HPAB-1277-FY) | ELISA, Neut, WB | Human IgG |
| HPAB-1278-FY | Human Anti-JEV Recombinant Antibody (HPAB-1278-FY) | ELISA, Neut, WB | Human IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-364, RRID: AB_3111585)
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