Rabbit Anti-KDM1B Recombinant Antibody (clone R08-5J5)
CAT#: VS3-XY3405
This antibody is a recombinant rabbit antibody binding with KDM1B (Lysine demethylase 1B). It specifically reacts with human, mouse and rat KDM1B. It has been detected using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunoprecipitation, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/20, 1/50-1/100 and 1/50-1/200, respectively.
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KDM1B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-5J5
- Applications
- WB, ICC, IF, IP, FC
- Related Disease
- Bainbridge-Ropers Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- AOF1; LSD2; C6orf193
- Gene ID
- 221656
- UniProt ID
- Q8NB78
- Long Name
- Lysine demethylase 1B
- Cellular Localization
- Nucleus, Chromosome
- Post Translation Modifications
- Ubiquitination at Lys649 and Lys378 (NX_Q8NB78 [NX_Q8NB78-1])
Modification sites at PhosphoSitePlus (Q8NB78)
Glycosylation from GlyGen (Q8NB78) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005248985.1
- Function
- Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
Required for de novo DNA methylation of a subset of imprinted genes during oogenesis.
Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Demethylates both mono- and di-methylated 'Lys-4' of histone H3.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KDM1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ60 | Mouse Anti-KDM1B Recombinant Antibody (VS3-CJ60) | WB, ELISA | Mouse IgG |
| VS4-WK264 | Rabbit Anti-KDM1B Recombinant Antibody (clone 15F3) | ELISA, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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