Rabbit Anti-KDM1B Recombinant Antibody (clone R08-5J5) (CAT#: VS3-XY3405)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat KDM1B

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R08-5J5

• Applications
• WB, ICC, IF, IP, FC

• Related Disease
• Bainbridge-Ropers Syndrome

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IF: 1/50-1/200
  IP: 1/20
  FC: 1/50-1/100

• Alternative Names
• AOF1; LSD2; C6orf193

• Gene ID
• 221656

• UniProt ID
• Q8NB78

• Long Name
• Lysine demethylase 1B

• Cellular Localization
• Nucleus, Chromosome

• Post Translation Modifications
• Ubiquitination at Lys649 and Lys378 (NX_Q8NB78 [NX_Q8NB78-1])
  Modification sites at PhosphoSitePlus (Q8NB78)
  Glycosylation from GlyGen (Q8NB78) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_005248985.1

• Function
• Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
  Required for de novo DNA methylation of a subset of imprinted genes during oogenesis.
  Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
  Demethylates both mono- and di-methylated 'Lys-4' of histone H3.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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