Rabbit Anti-KDM2A Recombinant Antibody (clone R05-4D6)
CAT#: VS3-FY1715
Clone R05-4D6 is a rabbit recombinant monoclonal antibody that is designed to target human KDM2A. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Western blot demonstrated that R05-4D6 recognized a target protein with a molecular weight of 133 kDa.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44251/if_selected.jpg
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Specifications
- Immunogen
- Synthetic peptide of human FBXL11
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KDM2A
- Species Reactivity
- Human
- Clone
- R05-4D6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 133 kDa, while the observed molecular weight stands at 133 kDa.
- Related Disease
- Duodenum Adenoma and Ossifying Fibromyxoid Tumor
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Lysine Demethylase 2A; F-Box And Leucine-Rich Repeat Protein 11; JmjC Domain-Containing Histone Demethylation Protein 1A; Jumonji C Domain-Containing Histone Demethylase 1A; [Histone-H3]-Lysine-36 Demethylase 1A; Lysine (K)-Specific Demethylase 2A; CXXC-Type Zinc Finger Protein 8; F-Box/LRR-Repeat Protein 11; EC 1.14.11.27; FBXL11; JHDM1A
- Gene ID
- 22992
- UniProt ID
- Q9Y2K7
- Sequence Similarities
- Belongs to the JHDM1 histone demethylase family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Mono-ADP-ribosylated at Arg-1020 in response to DNA damage, leading to displacement from chromatin, resulting in increased dimethylation of histone H3 at 'Lys-36'.
- Protein Refseq
- XP_006718543.1; XP_011543163.1; XP_016872880.1; XP_016872881.1; XP_047282579.1
- Function
- Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KDM2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-365 | Recombinant Human Anti-Human KDM2A Antibody, clone D435 | IF, ELISA, IP-MS | IgG |
| VS7-0425-WR195 | Mouse Anti-KDM2A Recombinant Antibody (clone 5C11F7) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3748 | Anti-KDM2A Immunohistochemistry Kit | IHC |
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