Mouse Anti-KDM5B Recombinant Antibody (clone 7H3D7) (CAT#: VS7-0425-WR203)
This product is a mouse IgG1 antibody that binds to human KDM5B. The antibody 7H3D7 recognizes specific amino acid domain of human KDM5B (AA: 231-319). It can be used for FC assay. It cross reacts with mouse.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53723/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53723/if_selected.jpg
Figure 2 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_6_6.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_B_5_1.jpg
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_B_5_1.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_8_3.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117139-KDM5B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117139-KDM5B
Specifications
- Immunogen
- Recombinant human KDM5B (AA: 231-319) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KDM5B
- Species Reactivity
- Human
- Clone
- 7H3D7
- Molecule Class
- Demethylase
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Intellectual developmental disorder, autosomal recessive 65 (MRT65)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM5B; Retinoblastoma-Binding Protein 2 Homolog 1; PLU-1; EC 1.14.11.-; Jumonji, AT Rich Interactive Domain 1B; Retinoblastoma-Binding Protein 2, Homolog 1A; RBBP2H1; Regulatory Subunit 98; Lysine-Specific Demethylase 5B; PLU1; Protein Phosphatase 1, Regu
- Gene ID
- 10765
- UniProt ID
- Q9UGL1
- Long Name
- Lysine demethylase 5B
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001300971.1
- Function
- Chromatin regulator; Dioxygenase; Oxidoreductase; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4070z | Mouse Anti-KDM5B Recombinant Antibody (clone 25F5) | WB, IP, FC, ELISA | Mouse IgG1 |
| MOB-1433MZ | Recombinant Mouse Anti-Human KDM5B Antibody | ICC/IF, IHC-P, WB | Mouse antibody |
| MRO-1992-CN | Rabbit Anti-KDM5B Polyclonal Antibody (MRO-1992-CN) | IF, IHC | Rabbit IgG |
| PABS-0094 | Camelid Anti-KDM5B Recombinant Single Domain Antibody (Clone NB17) | ELISA, FuncS | Camelid VHH |
| VS3-CJ44 | Mouse Anti-KDM5B Recombinant Antibody (VS3-CJ44) | WB, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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