Mouse Anti-KDM5B Recombinant Antibody (clone 7H3D7) (CAT#: VS7-0425-WR203)
This product is a mouse IgG1 antibody that binds to human KDM5B. The antibody 7H3D7 recognizes specific amino acid domain of human KDM5B (AA: 231-319). It can be used for FC assay. It cross reacts with mouse.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53723/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53723/if_selected.jpg
Figure 2 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_6_6.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_B_5_1.jpg
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_B_5_1.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68193/152066_A_8_3.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117139-KDM5B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117139-KDM5B
Specifications
- Immunogen
- Recombinant human KDM5B (AA: 231-319) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KDM5B
- Species Reactivity
- Human
- Clone
- 7H3D7
- Molecule Class
- Demethylase
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Intellectual developmental disorder, autosomal recessive 65 (MRT65)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM5B; Retinoblastoma-Binding Protein 2 Homolog 1; PLU-1; EC 1.14.11.-; Jumonji, AT Rich Interactive Domain 1B; Retinoblastoma-Binding Protein 2, Homolog 1A; RBBP2H1; Regulatory Subunit 98; Lysine-Specific Demethylase 5B; PLU1; Protein Phosphatase 1, Regu
- Gene ID
- 10765
- UniProt ID
- Q9UGL1
- Long Name
- Lysine demethylase 5B
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001300971.1
- Function
- Chromatin regulator; Dioxygenase; Oxidoreductase; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4070z | Mouse Anti-KDM5B Recombinant Antibody (clone 25F5) | WB, IP, FC, ELISA | Mouse IgG1 |
| MOB-1433MZ | Recombinant Mouse Anti-Human KDM5B Antibody | ICC/IF, IHC-P, WB | Mouse antibody |
| MRO-1992-CN | Rabbit Anti-KDM5B Polyclonal Antibody (MRO-1992-CN) | IF, IHC | Rabbit IgG |
| PABS-0094 | Camelid Anti-KDM5B Recombinant Single Domain Antibody (Clone NB17) | ELISA, FuncS | Camelid VHH |
| VS3-CJ44 | Mouse Anti-KDM5B Recombinant Antibody (VS3-CJ44) | WB, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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