Mouse Anti-KLHL22 Monoclonal Antibody (VS7-0425-WR235)
CAT#: VS7-0425-WR235
This product is a mouse IgG antibody that binds to human KLHL22. The antibody can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HAP1
Immunofluorescent staining of human cell line HAP1 shows localization to cytokinetic bridge & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40766/1852_E5_33_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000099910-KLHL22
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Specifications
- Immunogen
- Recombinant human KLHL22 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KLHL22
- Species Reactivity
- Human
- Molecule Class
- Kelch-Like Protein
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- isolated coloboma
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KELCHL
- Gene ID
- 84861
- UniProt ID
- Q53GT1
- Long Name
- Kelch Like Family Member 22
- Cellular Localization
- Cytoplasm; Cytoskeleton; Lysosome; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_116164.2
- Function
- Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478).; The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KLHL22"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0250C | Mouse Anti-KLHL22 Recombinant Antibody (ZG-0250C) | WB, IF, ELISA | Mouse IgG |
| VS3-XY984 | Mouse Anti-KLHL22 Recombinant Antibody (clone 7B10) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1195 | Anti-KLHL22 Immunohistochemistry Kit | IHC |
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