Mouse Anti-KLHL22 Monoclonal Antibody (VS7-0425-WR235) (CAT#: VS7-0425-WR235)

• Immunogen
• Recombinant human KLHL22 fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human KLHL22

• Species Reactivity
• Human

• Molecule Class
• Kelch-Like Protein

• Applications
• WB, IF, ICC, ELISA

• Conjugate
• Unconjugated

• Related Disease
• isolated coloboma

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• 1 mg/ml

• Preservative
• 0.03% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunofluorescence: 1:200 - 1:1000
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• KELCHL

• Gene ID
• 84861

• UniProt ID
• Q53GT1

• Long Name
• Kelch Like Family Member 22

• Cellular Localization
• Cytoplasm; Cytoskeleton; Lysosome; Nucleus

• Post Translation Modifications
• Acetylation; Phosphoprotein

• Protein Refseq
• NP_116164.2

• Function
• Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478).; The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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