Mouse Anti-KRT14 Recombinant Antibody (clone ABT-CK14)
CAT#: VS3-CJ290
This product is a mouse antibody that recognizes KRT14.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/1660_C10_5_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_8_5.jpg
Normal Tissue
Figure 4 Skin
Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/134/1050_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT, hTCEpi, hTERT-HME1, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186847-KRT14
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Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 14
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT14
- Species Reactivity
- Human
- Clone
- ABT-CK14
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- Keratin type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; NFJ; CK14; EBS3; EBS4
- Gene ID
- 3861
- UniProt ID
- P02533
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
- Protein Refseq
- NP_000517.2
- Function
- The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1120MZ | Chicken Anti-Keratin 14 Polyclonal IgY | IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1978 | Hi-Affi™ Recombinant Rabbit Anti-KRT14 Monoclonal Antibody (DS1978AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0234J | Mouse Anti-KRT14 Recombinant Antibody (ZG-0234J) | IHC-P, IF | Mouse IgG |
| ZG-0235J | Mouse Anti-KRT14 Recombinant Antibody (ZG-0235J) | IHC-P, IF | Mouse IgG |
| ZG-1080F | Mouse Anti-KRT14 Recombinant Antibody (clone RCK107) | FC, ICC, IHC-Fr, WB | Mouse IgG1 |
| ZG-1105F | Mouse Anti-KRT14 Recombinant Antibody (clone SPK-14) | FC, ICC, IHC-Fr, WB | Mouse IgG1 |
| ZG-0320U | Rabbit Anti-KRT14 Recombinant Antibody (clone D19-N) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1380 | AbPlus™ Anti-KRT14 Magnetic Beads (VS-0724-YC1380) | IP, Protein Purification | |
| VS-0724-YC1431 | AbPlus™ Anti-Krt14 Magnetic Beads (VS-0724-YC1431) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1209 | Anti-KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3843 | Anti-Mouse KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3842 | Anti-Human KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3844 | Anti-Rat KRT14 Immunohistochemistry Kit | IHC |
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