Mouse Anti-KRT15 Monoclonal Antibody (VS7-0425-WR250)
CAT#: VS7-0425-WR250
This product is a mouse IgG antibody that binds to human KRT15. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23910/1320_G5_6_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24554/1320_E5_3_selected.jpg
Normal Tissue
Figure 3 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23910/53953_B_4_1_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24554/53979_A_7_3.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171346-KRT15
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Specifications
- Immunogen
- Recombinant human KRT15 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT15
- Species Reactivity
- Human
- Molecule Class
- Type I Cytokeratin Protein
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- KRT15; CK-15; Keratin-15, Basic; Keratin, Type I Cytoskeletal 15; Beta; Keratin 15; K1CO; KRTB; Keratin-15, Beta; Cytokeratin 15; Type I Cytoskeletal 15; CK15; Basic; Keratin 15, Type I
- Gene ID
- 3866
- UniProt ID
- P19012
- Long Name
- Keratin 15
- Cellular Localization
- Intermediate filament; Keratin
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_002266.3
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT15"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2279z | Mouse Anti-KRT15 Recombinant Antibody (clone 39A5) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0236J | Mouse Anti-KRT15 Recombinant Antibody (ZG-0236J) | IHC-P, IF | Mouse IgG |
| VS3-FY814 | Recombinant Mouse Anti-KRT15 Antibody (clone 1B6A9) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY815 | Recombinant Mouse Anti-KRT15 Antibody (clone 2G8-10H10-8C9) | IHC-P | Mouse IgG1 |
| VS3-XY3411 | Rabbit Anti-KRT15 Recombinant Antibody (clone R06-2F5) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1979 | Hi-Affi™ Recombinant Rabbit Anti-KRT15 Monoclonal Antibody (DS1979AB) | FC, WB | IgG |
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