Mouse Anti-KRT19 Recombinant Antibody (ZG-0464F)
CAT#: ZG-0464F
This product is a mouse antibody that recognizes the epitope AA 80-400 of the KRT19.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-KRT19 Recombinant Antibody (ZG-0464F) in WB.
Western blot analysis of truncated KRT19-His recombinant protein (1) and full-length KRT19(aa1-400)-hIgGFc transfected CHO-K1 cell lysates (2) using cytokeratin 19 monoclonal antibody.
IHC
Figure 2 Mouse Anti-KRT19 Recombinant Antibody (ZG-0464F) in IHC.
Immunohistochemical analysis of paraffin-embedded human breast cancer (A), lung cancer (B) and normal colon tissue (C), using cytokeratin 19 monoclonal antibody to show cytoplasmic localization by DAB staining.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/1462_C1_1_selected.jpg
Normal Tissue
Figure 2 Urinary bladder
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_5_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_B_3_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HBEC3-KT, MCF7, RT4, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171345-KRT19
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Specifications
- Immunogen
- Recombinant fragment of Cytokeratin 19 (aa80-400) expressed in
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- E. coli KRT19
- Species Reactivity
- E. coli, Human
- Applications
- Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
ELISA: 1/10000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KRT19"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1582z | Mouse Anti-KRT19 Recombinant Antibody (clone 36D1) | WB, FC, ICC, IHC | Mouse IgG1 |
| ZG-0324U | Rabbit Anti-KRT19 Recombinant Antibody (clone E16-L) | IHC-P | Rabbit IgG |
| VS3-CJ85 | Mouse Anti-KRT19 Recombinant Antibody (VS3-CJ85) | WB, ELISA | Mouse IgG |
| VS3-FY822 | Recombinant Mouse Anti-KRT19 Antibody (clone 5A8-10E7-4B7) | IHC-P | Mouse IgG1 |
| VS3-FY823 | Recombinant Rabbit Anti-KRT19 Antibody (clone R07-1D4) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1983 | Hi-Affi™ Recombinant Rabbit Anti-KRT19 Monoclonal Antibody (DS1983AB) | FC, ICC, IHC-P, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN-F(E) | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN-S(P) | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1429 | AbPlus™ Anti-KRT19 Magnetic Beads (VS-0724-YC1429) | IP, Protein Purification | |
| VS-0724-YC1430 | AbPlus™ Anti-KRT19 Magnetic Beads (VS-0724-YC1430) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1214 | Anti-KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3862 | Anti-Mouse KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3864 | Anti-Rat KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3861 | Anti-Human KRT19 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC635 | CytoStream™ Rabbit Anti-KRT19 Polyclonal Antibody (VS13-YC635) | WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF | Rabbit IgG |
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