Mouse Anti-KRT20 Recombinant Antibody (clone 2C7B9) (CAT#: VS7-0425-WR264)
This product is a mouse IgG1 antibody that binds to human KRT20. The antibody 2C7B9 recognizes specific amino acid domain of human KRT20 (AA: 1-150). It can be used for IHC assay.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24684/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24684/if_selected.jpg
Figure 2 High expression in small intestine
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_5_2_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_5_2_val_selected.jpg
Figure 3 Low expression in liver
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_7_4_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_7_4_val_selected.jpg
Figure 4 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32/127_A_8_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32/127_A_8_3.jpg
Figure 5 RNA cell line category: Cell line enhanced (NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171431-KRT20
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171431-KRT20
Specifications
- Immunogen
- Recombinant human KRT20 (AA: 1-150) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KRT20
- Species Reactivity
- Human
- Clone
- 2C7B9
- Molecule Class
- Keratin Family
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- K20; CD20; CK20; CK-20; KRT21
- Gene ID
- 54474
- UniProt ID
- P35900
- Long Name
- Keratin 20
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_061883.1
- Function
- Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1373z | Mouse Anti-KRT20 Recombinant Antibody (clone 27D4) | WB, ICC, IF, FC, ELISA | Mouse IgG2a |
| MRO-0444-CN | Recombinant Rabbit Anti-KRT20 Monoclonal Antibody (CBACN-182) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ910 | Mouse Anti-KRT20 Recombinant Antibody (clone ABT-Ck20) | IHC-P | Mouse IgG2a, κ |
| VS3-CJ911 | Rabbit Anti-KRT20 Recombinant Antibody (VS3-CJ911) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY825 | Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9) | IHC-P | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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