Mouse Anti-KRT5/KRT6 Recombinant Antibody (VS3-CJ638) (CAT#: VS3-CJ638)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 5/6

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human KRT5/6

• Species Reactivity
• Human

• Applications
• IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.2% BSA, PBS.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunohistochemistry (1:100-1:300).

• Alternative Names
• K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A; K6A; K6C; K6D; PC3; CK6A; CK6C; CK6D; CK-6C; CK-6E; KRT6C; KRT6D; K6B; PC2; PC4; CK6B; CK-6B; KRTL1

• Gene ID
• 3852

• UniProt ID
• P13647

• Sequence Similarities
• Belongs to the intermediate filament family.

• Cellular Localization
• Intermediate filament, Keratin

• Post Translation Modifications
• Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle (By similarity).
  Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis (By similarity).
  CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury (By similarity).

• Protein Refseq
• NP_000415.2

• Function
• Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS