Rabbit Anti-KRT7 Recombinant Antibody (clone R09-7D1)
CAT#: VS3-XY3412
This product is a recombinant rabbit antibody that recognizes human KRT7 (Keratin 7), which blocks interferon-dependent interphase and stimulates DNA synthesis in cells. KRT7 involves in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). The antibody also has cross-reactivity against mouse and rat KRT7. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KRT7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-7D1
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Bile Duct Adenoma; Cystadenoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- K7; CK7; SCL; K2C7
- Gene ID
- 3855
- UniProt ID
- P08729
- Long Name
- Keratin 7
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Protein Refseq
- NP_005547.3
- Function
- Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3412. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1992 | Hi-Affi™ Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (DS1992AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0152J | Mouse Anti-KRT7 Recombinant Antibody (clone 12D7) | IF, ICC, WB, IHC-P, IP | Mouse IgG |
| ZG-0256J | Mouse Anti-KRT7 Recombinant Antibody (ZG-0256J) | IHC-P, IF | Mouse IgG |
| ZG-0257J | Mouse Anti-KRT7 Recombinant Antibody (ZG-0257J) | IHC-P, IF | Mouse IgG |
| VS4-CJ12 | Recombinant Mouse Anti-KRT7 Antibody (VS4-CJ12) | ELISA, WB, IHC, IF, IP | Mouse IgG |
| VS4-CJ13 | Recombinant Mouse Anti-KRT7 Antibody (VS4-CJ13) | ELISA, WB, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1383 | AbPlus™ Anti-KRT7 Magnetic Beads (VS-0724-YC1383) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3884 | Anti-KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3885 | Anti-Mouse KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3887 | Anti-Rat KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3888 | Anti-Canine KRT7 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, WB, BLI, SPR
Application: WB, IF, FuncS
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, FC, Neut, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
