Mouse Anti-KRT7 Recombinant Antibody (ZG-0470F)
CAT#: ZG-0470F
This product is a mouse antibody that recognizes the epitope N-terminus of the KRT7.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-KRT7 Recombinant Antibody (ZG-0470F) in WB.
Western blot analysis was performed using cytokeratin 7 monoclonal antibodies against A459 and HeLa cell lysates.
IF
Figure 2 Mouse Anti-KRT7 Recombinant Antibody (ZG-0470F) in IF.
Immunofluorescence analysis of HeLa cells using cytokeratin 7 monoclonal antibody.
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
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Specifications
- Immunogen
- Recombinant human Cytokeratin 7 (N-terminus) protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human KRT7
- Species Reactivity
- Human, Mouse, Rat, Bovine
- Applications
- Western Blot, Immunofluorescence
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/1000-1/2000
Immunofluorescence: 1/100-1/500
Target
- Alternative Names
- Keratin 7; Sarcolectin; Keratin, 55K Type II Cytoskeletal; Keratin, Type II Cytoskeletal 7; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; CK-7; SCL; KRT7; SCL; Keratin; type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin- 7; K7; Sarcolectin; Type-II keratin Kb7
- Gene ID
- 3855
- UniProt ID
- P08729
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KRT7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1300z | Mouse Anti-KRT7 Recombinant Antibody (clone 21C11) | FC, ICC, IF, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0301MZ | Recombinant Mouse Anti-Human Cytokeratin 7 Antibody (clone OW-TM 13/31) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1992 | Hi-Affi™ Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (DS1992AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0451-CN | Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (CBACN-188) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0455-CN | Recombinant Mouse Anti-KRT7 Monoclonal Antibody (3-G3-D8) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1807-CN | Rabbit Anti-KRT7 Polyclonal Antibody (MRO-1807-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1383 | AbPlus™ Anti-KRT7 Magnetic Beads (VS-0724-YC1383) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3884 | Anti-KRT7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3885 | Anti-Mouse KRT7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3887 | Anti-Rat KRT7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3888 | Anti-Canine KRT7 Immunohistochemistry Kit | IHC |
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