Mouse Anti-KRT8 Monoclonal Antibody (VS7-0425-WR272)
CAT#: VS7-0425-WR272
This product is a mouse IgG antibody that binds to human KRT8. The antibody recognizes specific amino acid domain of human KRT8 (aa391-483). It can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
Normal Tissue
Figure 8 Prostate
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
❮
❯
Specifications
- Immunogen
- Recombinant human KRT8 (aa391-483) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT8
- Species Reactivity
- Human
- Molecule Class
- Type I Intermediate Filament Protein
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cirrhosis (CIRRH)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Immunohistochemistry: 1:200 - 1:1000
Target
- Alternative Names
- KRT8; keratin 8, type II; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
- Gene ID
- 3856
- UniProt ID
- Q7L4M3
- Long Name
- Keratin 8
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001243211.1
- Function
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1808-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1808-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1809-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1809-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0471F | Mouse Anti-KRT8 Recombinant Antibody (ZG-0471F) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-CJ425 | Mouse Anti-KRT8 Recombinant Antibody (clone ABT-CK8) | WB, IHC-P | Mouse IgG1, κ |
| VS3-CJ426 | Mouse Anti-KRT8 Recombinant Antibody (clone PT1903) | WB, IHC-P | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0344JF | Human Anti-KRT8 Recombinant Antibody (clone COU-1) | WB, ELISA, IHC, FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1415 | AbPlus™ Anti-KRT8 Magnetic Beads (VS-0724-YC1415) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3897 | Anti-KRT8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3898 | Anti-Mouse KRT8 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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