Rabbit Anti-KRT8 Recombinant Antibody (clone R01-9E6)
CAT#: VS3-XY3413
It is a recombinant rabbit antibody that recognizes KRT8 (Keratin 8). It is capable of recognizing human and mouse KRT8. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
Normal Tissue
Figure 8 Prostate
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
❮
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse KRT8
- Species Reactivity
- Human, Mouse
- Clone
- R01-9E6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Cryptogenic Cirrhosis; Hidrocystoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
- Gene ID
- 3856
- UniProt ID
- P05787
- Long Name
- Keratin 8
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001243222.1; NP_002264.1
- Function
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1511MZ | Recombinant Mouse Anti-Human KRT8 Antibody (clone 2C23) | ICC, IF, WB | Mouse antibody |
| MRO-1808-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1808-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1809-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1809-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0471F | Mouse Anti-KRT8 Recombinant Antibody (ZG-0471F) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-QX665 | Mouse Anti-KRT8 Recombinant Antibody (clone 2F4-H9-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0344JF | Human Anti-KRT8 Recombinant Antibody (clone COU-1) | WB, ELISA, IHC, FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1415 | AbPlus™ Anti-KRT8 Magnetic Beads (VS-0724-YC1415) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3897 | Anti-KRT8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3898 | Anti-Mouse KRT8 Immunohistochemistry Kit | IHC |
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