Rabbit Anti-LAT (pY200) Recombinant Antibody (clone R07-3C5)
CAT#: VS3-WK2002
This product is a rabbit recombinant antibody that is specific to phosphorylated LAT. The target of this antibody is phospho-LAT (Tyr200). The clone R07-3C5 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of LAT (pTyr200) observed in WB experiments using this antibody is 36-38 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/1770_C1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
High expression. RNA expression: 29.0 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Low expression. RNA expression: 11.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_B_8_5_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human lymph node
High expression. RNA expression: 65.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2223/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_4_6.jpg
Normal Tissue
Figure 9 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2223/5874_B_8_4.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HEL, HMC-1, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213658-LAT
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WB
Figure 1 Rabbit Anti-LAT (pTyr200) Recombinant Antibody (clone R07-3C5) in WB.
Western blot analysis of phospho-LAT (Tyr200) in Jurkat lysates using anti-LAT (pTyr200) antibody, Cat# VS3-WK2002.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Tyr200 of human LAT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr200 of human LAT
- Species Reactivity
- Human
- Clone
- R07-3C5
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0165-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (A1) | ELISA | Human Fab |
| HPAB-J0166-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (C10) | ELISA | Human Fab |
| HPAB-J0167-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (D3) | ELISA | Human Fab |
| HPAB-J0168-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (G10) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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