Rabbit Anti-LGI1 Recombinant Antibody (clone R01-1H2)
CAT#: VS3-FY2020
The R01-1H2 recombinant monoclonal antibody firmly attaches to human LGI1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. The LGI1 antibody is recommended for detection of target protein by western blot analysis of Lgi1/EPT in mouse brain, rat brain lysates and western blot analysis of Lgi1/EPT in Hela, A549 lysates.
Gene Expression
Tested Data
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (AF22, CACO-2, NTERA-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108231-LGI1
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WB
Figure 1 Anti-LGI1 Antibody (VS3-FY2020) in WB.
The Lgi1/EPT expression in mouse brain, rat brain lysates were detected using the anti-LGI1 by western blot assay.
WB
Figure 2 Anti-LGI1 Antibody (VS3-FY2020) in WB.
The Lgi1/EPT expression in Hela, A549 lysates were detected using the anti-Lgi1/EPT by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Lgi1/EPT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LGI1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1H2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 64 kDa, while the observed molecular weight stands at 64 kDa.
- Related Disease
- Epilepsy, Familial Temporal Lobe, 1 and Autosomal Dominant Epilepsy With Auditory Features
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- LGI1; ADLTE; ADPAEF; ADPEAF; EPITEMPIN; EPT; ETL1; IB1099; leucine-rich; glioma inactivated 1; leucine rich glioma inactivated 1
- Gene ID
- 9211
- UniProt ID
- O95970
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Secreted, Synapse
- Post Translation Modifications
- Glycosylated. (O95970-LGI1_HUMAN)
Glycosylation at Asn192, Asn277, and Asn422 (NX_O95970 [NX_O95970-1])
Modification sites at PhosphoSitePlus (O95970)
Glycosylation from GlyGen (O95970) 4 sites
- Protein Refseq
- XP_016872400.1; XP_016872401.1; XP_054223117.1; XP_054223118.1; NP_001295204.1
- Function
- Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LGI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3366z | Mouse Anti-LGI1 Recombinant Antibody (clone 39H5) | WB, ICC, IF, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2046 | Hi-Affi™ Recombinant Rabbit Anti-LGI1 Monoclonal Antibody (DS2046AB) | WB, IHC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4008 | Anti-LGI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4009 | Anti-Mouse LGI1 Immunohistochemistry Kit | IHC |
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