Rabbit Anti-LIG1 Recombinant Antibody (clone R02-5A2)
CAT#: VS3-FY2021
The R02-5A2 recombinant monoclonal antibody is able to identify human LIG1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This LIG1 antibody has been teted positive by western blot analysis of LIG1 in PC-12 lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41431/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48071/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/671_E8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41431/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_B_5_1_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_A_7_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37015/75769_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105486-LIG1
❮
❯
WB
Figure 1 Anti-LIG1 Antibody (VS3-FY2021) in WB.
The LIG1 expression in PC-12 lysates were detected using the anti-LIG1 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human LIG1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat LIG1
- Species Reactivity
- Human, Rat
- Clone
- R02-5A2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 102 kDa, while the observed molecular weight stands at 102 kDa.
- Related Disease
- Immunodeficiency 96 and Bloom Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- LIGI; IMD96; hLig1
- Gene ID
- 3978
- UniProt ID
- P18858
- Sequence Similarities
- Belongs to the ATP-dependent DNA ligase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys226, Lys298, Lys407, and Lys795 (NX_P18858 [NX_P18858-1])
Modification sites at PhosphoSitePlus (P18858)
Glycosylation from GlyGen (P18858) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047294789.1; XP_047294790.1; XP_054176994.1; XP_054176995.1; NP_000225.1
- Function
- DNA ligase that seals nicks in double-stranded during DNA repair.
Also involved in DNA replication and DNA recombination.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2021. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LIG1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3111z | Mouse Anti-LIG1 Recombinant Antibody (clone 11B4) | ELISA, IHC, WB | Mouse IgG2a, κ |
| MOB-0068CT | Recombinant Mouse anti-Human LIG1 Monoclonal antibody (20I6) | FC, ICC, IF, IP, WB | |
| MRO-0480-CN | Recombinant Rabbit Anti-LIG1 Monoclonal Antibody (JB43-39) | WB, IHC | Rabbit IgG |
| ZG-0052U | Rabbit Anti-LIG1 Recombinant Antibody (clone 7B2) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2055 | Hi-Affi™ Recombinant Rabbit Anti-LIG1 Monoclonal Antibody (DS2055AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1243 | Anti-LIG1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4028 | Anti-Mouse LIG1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4027 | Anti-Human LIG1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA
-2.png)
Application: ELISA
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FuncS
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
