CytoStream™ Rabbit Anti-LIN28A Recombinant Antibody (VS13-YC662)
CAT#: VS13-YC662
The CytoStream™ Rabbit Anti-LIN28A Recombinant Antibody is specific for human LIN28A. LIN28A, part of LIN-28 family, regulates developmental timing, interacting with target mRNAs and miRNAs, like LET7. It influences stem cell processes and cancer progression. The anti-LIN28A antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68814/1783_E7_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
High expression. RNA expression: 5.2 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_6_6_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Low expression. RNA expression: 0.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_B_5_3_val_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_9_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_1_7.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (NTERA-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131914-LIN28A
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Specifications
- Immunogen
- Recombinant human LIN28.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LIN28A
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Embryonal Tumor With Multilayered Rosettes and Central Nervous System Primitive Neuroectodermal Neoplasm
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LIN28A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1638MZ | Recombinant Mouse Anti-Human LIN28A Antibody (clone 66DU69.23.2) | ELISA, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2060 | Hi-Affi™ Recombinant Rabbit Anti-LIN28A Monoclonal Antibody (DS2060AB) | FC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0925-CN | Recombinant Rabbit Anti-LIN28A Monoclonal Antibody (JA10-63) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0926-CN | Recombinant Mouse Anti-LIN28A Monoclonal Antibody (A4-A6) | WB, IF, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX690 | Mouse Anti-LIN28A Recombinant Antibody (clone A4-A6) | WB, ICC, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX691 | Mouse Anti-LIN28A Recombinant Antibody (clone 6D1F9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4037 | Anti-LIN28A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4038 | Anti-Mouse LIN28A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4039 | Anti-Rat LIN28A Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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