Rabbit Anti-LLGL1 Recombinant Antibody (clone R05-0B8)
CAT#: VS3-FY2024
The anti-LLGL1 antibody detected positive in western blot analysis of LLGL1 in A549, U2OS lysates. The recombinant monoclonal antibody demonstrated a greater affinity with human LLGL1. It is suitable for use in a variety of assays, including WB, IP.
Tested Data
Gene Expression
WB
Figure 1 Anti-LLGL1 Antibody (VS3-FY2024) in WB.
The LLGL1 expression in A549, U2OS lysates were detected using the anti-LLGL1 by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_2_7_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22924/58806_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131899-LLGL1
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Specifications
- Immunogen
- Recombinant human LLGL1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LLGL1
- Species Reactivity
- Human
- Clone
- R05-0B8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 115 kDa, while the observed molecular weight stands at 115 kDa.
- Related Disease
- Smith-Magenis Syndrome and Alzheimer Disease 12
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- LLGL1; DLG4; HUGL; HUGL-1; HUGL1; LLGL; Lgl1; Mgl1; scribble cell polarity complex component; LLGL scribble cell polarity complex component 1
- Gene ID
- 3996
- UniProt ID
- Q15334
- Sequence Similarities
- Belongs to the WD repeat L(2)GL family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Endosome, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated at least at Ser-663 by PRKCI. (Q15334-L2GL1_HUMAN)
Ubiquitination at Lys19, Lys27, Lys293, Lys502, and Lys780 (NX_Q15334 [NX_Q15334-1])
Modification sites at PhosphoSitePlus (Q15334)
Glycosylation from GlyGen (Q15334) 1 site, 2 O-linked glycans (1 site)
- Protein Refseq
- XP_011522151.1; XP_011522152.1; XP_011522153.1; XP_011522154.1; XP_011522155.1
- Function
- Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LLGL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2064 | Hi-Affi™ Recombinant Rabbit Anti-LLGL1 Monoclonal Antibody (DS2064AB) | ELISA, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4047 | Anti-LLGL1 Immunohistochemistry Kit | IHC |
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