Recombinant Mouse Anti-LNPK Monoclonal Antibody (11-1)
CAT#: MRO-0935-CN
This antibody is a mouse monoclonal antibody that is capable of binding to LNPK.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/105_H11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_A_6_6.jpg
Normal Tissue
Figure 7 Skin
Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_B_9_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14205/32956_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144320-LNPK
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse
- Species Reactivity
- Mouse, Rat
- Clone
- 11-1
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Exceptional Specificity in LNPK Targeting
The recombinant mouse anti-LNPK monoclonal antibody demonstrates unparalleled specificity in LNPK targeting, significantly enhancing the accuracy of cellular localization studies. An indispensable tool for precise endoplasmic reticulum research.
Creative Biolabs' Reliable Quality
Creative Biolabs' recombinant mouse anti-LNPK monoclonal antibody consistently delivers reliable and reproducible results. Its superior binding affinity greatly aids in the structural analysis of the nuclear envelope.
Q&As
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What is the recommended storage condition for the recombinant mouse anti-LNPK monoclonal antibody?
A: The recombinant mouse anti-LNPK monoclonal antibody should be stored at -20°C to ensure its stability and activity. For short-term storage, it can be kept at 4°C. Avoid repeated freeze-thaw cycles to maintain the antibody's integrity.
-
How should the antibody be prepared for use in experiments?
A: Before use, thaw the antibody completely on ice. Gently mix the solution without vortexing. Dilute the antibody in the appropriate buffer for the specific application. Ensure all reagents and samples are at the same temperature to avoid precipitation.
-
What applications are suitable for the recombinant mouse anti-LNPK monoclonal antibody?
A: This antibody is suitable for various applications, including Western blot, immunofluorescence, immunohistochemistry, and ELISA. It is designed to specifically bind to the LNPK protein, making it a versatile tool for different experimental setups.
-
How can the specificity of the recombinant mouse anti-LNPK monoclonal antibody be verified?
A: The specificity of this antibody can be verified through a control experiment using LNPK knockdown or knockout samples. Comparing the antibody's reactivity in treated versus untreated samples will demonstrate its specificity.
-
Are there any recommended positive control samples for this antibody?
A: Liver and kidney tissue lysates are recommended as positive control samples due to their high expression of LNPK. Using these samples can help verify the antibody's effectiveness and specificity in various assays.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "LNPK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0934-CN | Recombinant Mouse Anti-LNPK Monoclonal Antibody (7-3) | IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4066 | Anti-LNPK Immunohistochemistry Kit | IHC |
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