Mouse Anti-MAP1LC3B Monoclonal Antibody (clone 5H12)
CAT#: VS7-0425-WR330
This product is a mouse IgG1 antibody that binds to human MAP1LC3B. The antibody 5H12 can be used for FC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53767/965_A12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140941-MAP1LC3B
❮
❯
Specifications
- Immunogen
- Recombinant human MAP1LC3B fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MAP1LC3B
- Species Reactivity
- Human
- Clone
- 5H12
- Molecule Class
- Microtubule-Associated Protein
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
- Gene ID
- 81631
- UniProt ID
- Q9GZQ8
- Long Name
- Microtubule associated protein 1 light chain 3 beta
- Cellular Localization
- Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Membrane; Microtubule; Mitochondrion
- Post Translation Modifications
- Lipoprotein; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_073729.1
- Function
- Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329).; Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329).; In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758).; While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329).; Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205).; Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).; Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR330. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP1LC3B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4640 | Hi-Affi™ Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (TH153DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| MOR-0046-FY | Rabbit Anti-MAP1LC3B Recombinant Antibody (clone AFY0017) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0920-CN | Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (CBACN-344) | WB, IP | Rabbit IgG |
| MOB-0087F | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 9H5) | IHC-P, IF | Mouse IgG |
| VS3-QX711 | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 8H6-6D10-3A4) | IHC | Mouse IgG1 |
| VS3-QX712 | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 7B12-H3-C6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY3425 | Rabbit Anti-MAP1LC3B Recombinant Antibody (clone R06-4K9) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1051 | AbPlus™ Anti-MAP1LC3B Magnetic Beads (VS-0724-YC1051) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4197 | Anti-MAP1LC3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY4198 | Anti-Mouse MAP1LC3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY4199 | Anti-Rat MAP1LC3B Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: ELISA
-5.jpg)
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
-4.jpg)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
