Mouse Anti-MAP1LC3B Recombinant Antibody (clone 9H5)
CAT#: MOB-0087F
This product is a mouse antibody that recognizes MAP1LC3B.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-MAP1LC3B Recombinant Antibody (clone 9H5) (MOB-0087F) in IHC
The 1:200 dilution of LC3B mouse mAb was used for immunohistochemical analysis of paraffin-embedded human brain tissue.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53767/965_A12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140941-MAP1LC3B
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human MAP1LC3B
- Species Reactivity
- Human
- Clone
- 9H5
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
REVIEWS AND Q&AS
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Great Autophagy Marker
We used clone 9H5 to monitor autophagy in our cell lines via immunofluorescence. The antibody specifically recognized MAP1LC3B, allowing us to visualize autophagosome formation clearly. It is a key reagent for our autophagy studies.
Reliable In IHC-P
This mouse recombinant antibody performed well in immunohistochemistry on paraffin sections. It stained LC3B positive structures effectively, providing us with high-quality images for our tissue analysis of autophagic activity.
Q&As
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Is this antibody suitable for detecting autophagy markers?
A: Yes, this mouse antibody recognizes MAP1LC3B (LC3B), which is a central protein in the autophagy pathway and a widely used marker of autophagosomes. It is suitable for Immunohistochemistry on Paraffin Sections and Immunofluorescence.
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What is the clonality and host of this antibody?
A: This is a recombinant mouse monoclonal antibody, clone 9H5. It specifically binds to Human MAP1LC3B and is supplied as a liquid formulation for use in your research applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAP1LC3B"
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| MOR-4640 | Hi-Affi™ Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (TH153DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
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| MRO-0920-CN | Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (CBACN-344) | WB, IP | Rabbit IgG |
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| MRO-2008-CN | Rabbit Anti-MAP1LC3B Polyclonal Antibody (MRO-2008-CN) | WB, IF, IHC | Rabbit IgG |
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| VS3-QX711 | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 8H6-6D10-3A4) | IHC | Mouse IgG1 |
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| VS3-QX712 | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 7B12-H3-C6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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| VS3-WK1139 | Mouse Anti-MAP1LC3B Recombinant Antibody (VS3-WK1139) | WB, IHC, IF | Mouse IgG |
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| VS-0724-YC1051 | AbPlus™ Anti-MAP1LC3B Magnetic Beads (VS-0724-YC1051) | IP, Protein Purification |
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| VS-0525-XY4197 | Anti-MAP1LC3B Immunohistochemistry Kit | IHC |
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