Mouse Anti-MAP2K2 Monoclonal Antibody (VS7-0425-WR333)
CAT#: VS7-0425-WR333
This product is a mouse IgG antibody that binds to human MAP2K2. The antibody can be used for WB, IF, ICC, FC, and ELISA assays. It cross reacts with mouse and rat.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/640_C4_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/ihc_selected.jpg
Normal Tissue
Figure 5 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_B_4_8.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_8.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126934-MAP2K2
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Specifications
- Immunogen
- Recombinant human MAP2K2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAP2K2
- Species Reactivity
- Human, Mouse, Rat
- Molecule Class
- Map Kinase Kinase
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cardiofaciocutaneous syndrome 4 (CFC4)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CFC4; MEK2; MKK2; MAPKK2; PRKMK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Long Name
- Mitogen-Activated Protein Kinase Kinase 2
- Cellular Localization
- Cytoplasm; Membrane
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_109587.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2K2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX714 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9E8-7G2-5A10) | WB | Mouse IgG1 |
| VS3-QX715 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9G3-3H2-7B7) | WB | Mouse IgG1 |
| VS3-QX716 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 6F10-D7-G6) | WB | Mouse IgG1 |
| VS3-QX717 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 5C9-B8-D5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ915 | Mouse Anti-MAP2K2 Recombinant Antibody (VS3-CJ915) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1183 | AbPlus™ Anti-MAP2K2 Magnetic Beads (VS-0724-YC1183) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1293 | Anti-MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4209 | Anti-Mouse MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4208 | Anti-Human MAP2K2 Immunohistochemistry Kit | IHC |
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