Rabbit Anti-MAP2K2 Recombinant Antibody (clone R05-2G7) (CAT#: VS3-FY2033)
Clone R05-2G7 is a rabbit recombinant monoclonal antibody that is able to identify human MAP2K2. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This MEK2 antibody was verified by western blot analysis of MEK2 in mouse brain, mouse lung lysates and western blot analysis of MEK2 in Hela, A549 lysates.
Specific Inquiry
WB
Figure 1 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.
The MEK2 expression in mouse brain, mouse lung lysates were detected using the anti-MEK2 by western blot assay.
WB
Figure 2 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.
The MEK2 expression in Hela, A549 lysates were detected using the anti-MEK2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MEK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAP2K2
- Species Reactivity
- Human, Mouse
- Clone
- R05-2G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
- Related Disease
- Cardiofaciocutaneous syndrome (CFC syndrome)
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Phosphorylated by MAP2K1/MEK1 ().
(P36507-MP2K2_HUMAN)
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. (P36507-MP2K2_HUMAN)
Ubiquitination at Lys88 (NX_P36507 [NX_P36507-1])
Modification sites at PhosphoSitePlus (P36507)
Glycosylation from GlyGen (P36507) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_006722862.1; XP_047295056.1; XP_054177439.1; XP_054177440.1; NP_109587.1
- Function
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1130CT | Recombinant Mouse anti-Human MAP2K2 Monoclonal antibody (2B2) | IP, WB | |
| MRO-0980-CN | Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (CBACN-359) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MOB-0103F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 2C3) | WB | Mouse IgG |
| MOB-0104F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 7D11) | WB | Mouse IgG |
| VS3-CJ915 | Mouse Anti-MAP2K2 Recombinant Antibody (VS3-CJ915) | WB | Mouse IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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