Rabbit Anti-MAP2K2 Recombinant Antibody (clone R05-2G7)

CAT#: VS3-FY2033

Clone R05-2G7 is a rabbit recombinant monoclonal antibody that is able to identify human MAP2K2. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This MEK2 antibody was verified by western blot analysis of MEK2 in mouse brain, mouse lung lysates and western blot analysis of MEK2 in Hela, A549 lysates.

Gene Expression Tested Data
Figure 1 IF staining of human cell line MCF7 Figure 2 IF staining of human cell line A-431 Figure 3 IHC staining of human small intestine Figure 4 IHC staining of human stomach Figure 5 Skin Figure 6 Lymph node Figure 7 Liver Figure 8 RNA cell line category: Low cell line specificity
Figure 1 Anti-MAP2K2 Antibody (VS3-FY2033) in WB. Figure 2 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human MEK2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Mouse MAP2K2
  • Species Reactivity
  • Human, Mouse
  • Clone
  • R05-2G7
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
  • Related Disease
  • Cardiofaciocutaneous syndrome (CFC syndrome)

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2
  • Sequence Similarities
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
  • Cellular Localization
  • Cytoplasm, Membrane
  • Post Translation Modifications
  • MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Phosphorylated by MAP2K1/MEK1 ().
    (P36507-MP2K2_HUMAN)
    (Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. (P36507-MP2K2_HUMAN)
    Ubiquitination at Lys88 (NX_P36507 [NX_P36507-1])
    Modification sites at PhosphoSitePlus (P36507)
    Glycosylation from GlyGen (P36507) 5 sites, 1 O-linked glycan (5 sites)
  • Function
  • Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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