Rabbit Anti-MAP2K2 Recombinant Antibody (clone R05-2G7) (CAT#: VS3-FY2033)
Clone R05-2G7 is a rabbit recombinant monoclonal antibody that is able to identify human MAP2K2. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This MEK2 antibody was verified by western blot analysis of MEK2 in mouse brain, mouse lung lysates and western blot analysis of MEK2 in Hela, A549 lysates.
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WB
Figure 1 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.
The MEK2 expression in mouse brain, mouse lung lysates were detected using the anti-MEK2 by western blot assay.
WB
Figure 2 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.
The MEK2 expression in Hela, A549 lysates were detected using the anti-MEK2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MEK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAP2K2
- Species Reactivity
- Human, Mouse
- Clone
- R05-2G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
- Related Disease
- Cardiofaciocutaneous syndrome (CFC syndrome)
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Phosphorylated by MAP2K1/MEK1 ().
(P36507-MP2K2_HUMAN)
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. (P36507-MP2K2_HUMAN)
Ubiquitination at Lys88 (NX_P36507 [NX_P36507-1])
Modification sites at PhosphoSitePlus (P36507)
Glycosylation from GlyGen (P36507) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_006722862.1; XP_047295056.1; XP_054177439.1; XP_054177440.1; NP_109587.1
- Function
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
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