Rabbit Anti-MAP2K2 Recombinant Antibody (clone R05-2G7)
CAT#: VS3-FY2033
Clone R05-2G7 is a rabbit recombinant monoclonal antibody that is able to identify human MAP2K2. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This MEK2 antibody was verified by western blot analysis of MEK2 in mouse brain, mouse lung lysates and western blot analysis of MEK2 in Hela, A549 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
❮
❯
WB
Figure 1 Anti-MAP2K2 Antibody (VS3-FY2033) in WB.
The MEK2 expression in mouse brain, mouse lung lysates were detected using the anti-MEK2 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human MEK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAP2K2
- Species Reactivity
- Human, Mouse
- Clone
- R05-2G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
- Related Disease
- Cardiofaciocutaneous syndrome (CFC syndrome)
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Phosphorylated by MAP2K1/MEK1 ().
(P36507-MP2K2_HUMAN)
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. (P36507-MP2K2_HUMAN)
Ubiquitination at Lys88 (NX_P36507 [NX_P36507-1])
Modification sites at PhosphoSitePlus (P36507)
Glycosylation from GlyGen (P36507) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_006722862.1; XP_047295056.1; XP_054177439.1; XP_054177440.1; NP_109587.1
- Function
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2033. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2K2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1130CT | Recombinant Mouse anti-Human MAP2K2 Monoclonal antibody (2B2) | IP, WB | |
| VS3-QX714 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9E8-7G2-5A10) | WB | Mouse IgG1 |
| VS3-QX715 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9G3-3H2-7B7) | WB | Mouse IgG1 |
| VS7-0425-WR332 | Mouse Anti-MAP2K2 Monoclonal Antibody (clone 7F5) | WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR333 | Mouse Anti-MAP2K2 Monoclonal Antibody (VS7-0425-WR333) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1183 | AbPlus™ Anti-MAP2K2 Magnetic Beads (VS-0724-YC1183) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1293 | Anti-MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4209 | Anti-Mouse MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4208 | Anti-Human MAP2K2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
-2.png)
Application: ELISA, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
-CB2006C17L-4.jpg)
Application: WB, ELISA
Application: WB, IHC, ELISA, FC
Application: ELISA, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
