Rabbit Anti-MAP2K2 Recombinant Antibody (VS13-YC688)
CAT#: VS13-YC688
The Rabbit Anti-MAP2K2 Recombinant Antibody is specific for human, mouse and rat MAP2K2. MAP2K2, a kinase family member, phosphorylates MAPK1/ERK2 and MAPK2/ERK3, involved in signal transduction and syndromes like cardiofaciocutaneous with features like heart defects and cognitive disability. The anti-MAP2K2 antibody can be used for Western blot (1/1000), Immunocytochemistry (1/50), Immunofluorescence (1/50), Immunohistochemistry-paraffin (1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/640_C4_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/ihc_selected.jpg
Normal Tissue
Figure 5 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_B_4_8.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_8.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126934-MAP2K2
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Specifications
- Immunogen
- Recombinant human MEK1, aa 1-393.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP2K2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
- Related Disease
- cardiofaciocutaneous syndrome (CFC syndrome)
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2;
- Gene ID
- 5605
- UniProt ID
- P36507
- Long Name
- Mitogen-Activated Protein Kinase Kinase 2
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Nucleus; Cytoplasm; Mitochondrion; Cell membrane
- Protein Refseq
- XP_006722862.1; XP_047295056.1; XP_054177439.1; XP_054177440.1; NP_109587.1
- Function
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAP2K2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0103F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 2C3) | WB | Mouse IgG |
| MOB-0104F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 7D11) | WB | Mouse IgG |
| VS3-CJ915 | Mouse Anti-MAP2K2 Recombinant Antibody (VS3-CJ915) | WB | Mouse IgG |
| VS7-0425-WR332 | Mouse Anti-MAP2K2 Monoclonal Antibody (clone 7F5) | WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR333 | Mouse Anti-MAP2K2 Monoclonal Antibody (VS7-0425-WR333) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1183 | AbPlus™ Anti-MAP2K2 Magnetic Beads (VS-0724-YC1183) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1293 | Anti-MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4209 | Anti-Mouse MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4208 | Anti-Human MAP2K2 Immunohistochemistry Kit | IHC |
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