Mouse Anti-MAP2K4 Monoclonal Antibody (clone 2D10D8)
CAT#: VS7-0425-WR336
This product is a mouse IgM antibody that binds to human MAP2K4. The antibody 2D10D8 can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60074/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7751/18833_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7751/18833_A_5_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065559-MAP2K4
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Specifications
- Immunogen
- Recombinant human MAP2K4 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Human MAP2K4
- Species Reactivity
- Human
- Clone
- 2D10D8
- Molecule Class
- Ser/Thr Protein Kinase
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
- Gene ID
- 6416
- UniProt ID
- P45985
- Long Name
- Mitogen-Activated Protein Kinase Kinase 4
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001268364.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2K4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1459MZ | Recombinant Mouse Anti-Human MAP2K4 Antibody (clone 3E20E9,5H22C3) | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0278C | Mouse Anti-MAP2K4 Recombinant Antibody (ZG-0278C) | IHC, ELISA | Mouse IgG |
| VS7-0425-WR335 | Mouse Anti-MAP2K4 Monoclonal Antibody (clone 5H4) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR337 | Mouse Anti-MAP2K4 Monoclonal Antibody (VS7-0425-WR337) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| VS7-0425-WR338 | Mouse Anti-MAP2K4 Monoclonal Antibody (VS7-0425-WR338) | IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2134 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K4 Monoclonal Antibody (DS2134AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4211 | Anti-MAP2K4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4212 | Anti-Mouse MAP2K4 Immunohistochemistry Kit | IHC |
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