AbPlus™ Anti-MBD4 Magnetic Beads (VS-0724-YC156)
CAT#: VS-0724-YC156
The AbPlus Anti-MBD4 Magnetic Beads (VS-0724-YC156) is an innovative affinity resin which is bound with anti-MBD4 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of MBD4 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells and adnexal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/36_A1_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/37_A1_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_4_6.jpg
Normal Tissue
Figure 7 Skin
Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_7_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129071-MBD4
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-MBD4 specific antibody
- Target
- MBD4
- Immunogen
- Synthetic peptide corresponding to the center region of the human MBD4.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
- Alternative Names
- Methyl-CpG Binding Domain 4, DNA Glycosylase; Methyl-CpG Binding Domain Protein 4; Mismatch-Specific DNA N-Glycosylase; Methyl-CpG-Binding Endonuclease 1; Methyl-CpG-Binding Protein MBD4; MED1; G/5-Fluorouracil Mismatch Glycosylase With Biphasic Kinetics;
- Gene ID
- 8930
- UniProt ID
- O95243
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "MBD4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-399 | Recombinant Human Anti-Human MBD4 Antibody Fab Fragment, clone D84 | IP, IF, ELISA | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ106 | Mouse Anti-MBD4 Recombinant Antibody (VS3-CJ106) | WB, ELISA | Mouse IgG |
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