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    Mouse Anti-MBD4 Recombinant Antibody (VS3-CJ106) (CAT#: VS3-CJ106)

    This product is a mouse antibody that recognizes MBD4.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/if_selected.jpg
    Figure 2 IHC staining of human skin
    Figure 2 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells and adnexal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/ihc_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/36_A1_1_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/37_A1_1_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_4_6.jpg
    Figure 7 Skin
    Figure 7 Skin
    (Fibroblasts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Keratinocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Langerhans
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Melanocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_7_1.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129071-MBD4

    Specifications

    • Immunogen
    • Recombinant fragment of human MBD4 (N-terminus)
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human MBD4
    • Species Reactivity
    • Human
    • Applications
    • WB, ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Concentration
    • 1 mg/mL (lot specific)
    • Buffer
    • 50% Glycerol, 1% BSA, PBS, pH7.4.
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

    Target

    • Alternative Names
    • MED1; TPDS2
    • Cellular Localization
    • Nucleus
    • Function
    • Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "MBD4"

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    CAT Product Name Application Type
    DrMAB-399 Recombinant Human Anti-Human MBD4 Antibody Fab Fragment, clone D84 IP, IF, ELISA Fab

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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