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Mouse Anti-MBD4 Recombinant Antibody (VS3-CJ106) (CAT#: VS3-CJ106)

This product is a mouse antibody that recognizes MBD4.


See more Engineered Antibody products for MBD4
Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/if_selected.jpg
Figure 2 IHC staining of human skin
Figure 2 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells and adnexal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/36_A1_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/37_A1_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_4_6.jpg
Figure 7 Skin
Figure 7 Skin
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_B_7_1.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2031/8666_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129071-MBD4

Specifications

  • Immunogen
  • Recombinant fragment of human MBD4 (N-terminus)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human MBD4
  • Species Reactivity
  • Human
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL (lot specific)
  • Buffer
  • 50% Glycerol, 1% BSA, PBS, pH7.4.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

Target

  • Alternative Names
  • MED1; TPDS2
  • Cellular Localization
  • Nucleus
  • Function
  • Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "MBD4"

Fab Fragment Antibody

CAT Product Name Application Type
DrMAB-399 Recombinant Human Anti-Human MBD4 Antibody Fab Fragment, clone D84 IP, IF, ELISA Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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